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논문 기본 정보

자료유형
학술저널
저자정보
Oh, Hyun-Wook (Division of Food Bioscience and Technology, Korea University) Jeun, Gi-Hoon (Division of Food Bioscience and Technology, Korea University) Lee, Jin (Division of Food Bioscience and Technology, Korea University) Chun, Tae-Hoon (Division of Biotechnology, Korea University) Kim, Sae-Hun (Division of Food Bioscience and Technology, Korea University)
저널정보
한국축산식품학회 한국축산식품학회지 한국축산식품학회지 제32권 제4호
발행연도
2012.1
수록면
434 - 440 (7page)

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It has been suggested that probiotics could be useful for the prevention of symptomatic relapse in patients with inflammatory bowel disease (IBD). Interleukin (IL)-8 has been well recognized as one of the pro-inflammatory cytokines that could trigger inflammation and epithelial barrier dysfunction. In this study, the anti-inflammatory effects of probiotics were investigated using a human epithelial cell line (HT-29). Probiotics from infant feces and kimchi were tested for their cytotoxicity and effects on adhesion to epithelial cells. The present results show that seven strains could form 70 % adhesion on HT-29. The probiotics used in this study did not affect HT-29 cell viability. To screen anti-inflammatory lactic acid bacteria, HT-29 cells were pretreated with live and heat-killed probiotics, and lipopolysaccharide (LPS) ($1{\mu}g/mL$) was then added to stimulate the cells. The cell culture supernatant was then used to measure IL-8 secretion by ELISA, and the cell pellet was used to determine IL-8 and toll-like receptor (TLR-4) mRNA expression levels by RT-PCR. Some probiotics (KJP421, KDK411, SRK414, E4191, KY21, and KY210) exhibited anti-inflammatory effects through the repression of IL-8 secretion from HT-29 cells. In particular, Lactobacillus salivarius E4191, originating from Egyptian infant feces, not only decreased IL-8 mRNA expression, but also decreased TLR-4 expression. These results indicate that Lactobacillus salivarius E4191 may have a protective effect in intestinal epithelial cells.

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