지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 241 - 246 (6page)
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초록· 키워드
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This report outlines the development of a rapid, simple, and sensitive detection system for pathogenic bacteria using a capillary electrophoresis-based, single strand conformation polymorphism (CE-SSCP) combined with PCR. We demonstrate that this method, used with primers targeting the V4 region of the16S rRNA gene, is capable of the simultaneous detection of 10 microbes that could be associated with foodborne illness, caused by animal-derived foods: Salmonella enterica, Listeria monocytogenes, Escherichia coli O157:H7, Campylobacter jejuni, Staphylococcus aureus, Bacillus cereus, Clostridium perfringens, Yersinia enterocolitica, Vibrio parahaemolyticus, and Enterobacter sakazakii. The traditional detection techniques are time-consuming and labor-intensive, due to the necessary task of separate cultivation of each target species. As such, the CE-SSCP-PCR method, that we have developed, has the potential to diagnose pathogens rapidly, unlike the traditional technique, in order to prevent foodborne illness in a much more efficient manner.
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참고문헌 (19)
참고문헌 신청
Altekruse, S. F. / 1999 / Campylobacter jejuni – An emerging foodborne pathogen / Emerging Infectious Diseases 5:28~35
Andersen, P. S. / 2003 / Capillary electrophoresis-based single strand DNA conformation analysis in high-throughput mutation screening / Human Mutation 21:455~465
Animal Plant and Fisheries Quarantine and Inspection Agency / Standard for processing and ingredients of specifications livestock products
Claesson, M. J. / 2010 / Comparison of two next-generation sequencing technologies for resolving highly complex microbiota composition using tandem variable 16S rRNA gene regions / Nucleic Acids Research 38:1~13
Gillman, L. M. / 2001 / Identification of Mycobacterium species by multiple-fluorescence PCR-single-strand conformation polymorphism analysis of the 16S rRNA gene / Journal of Clinical Microbiology 39:3085~3091
Andersen, P. S. / 2003 / Capillary electrophoresis-based single strand DNA conformation analysis in high-throughput mutation screening / Human Mutation 21:455~465
Animal Plant and Fisheries Quarantine and Inspection Agency / Standard for processing and ingredients of specifications livestock products
Claesson, M. J. / 2010 / Comparison of two next-generation sequencing technologies for resolving highly complex microbiota composition using tandem variable 16S rRNA gene regions / Nucleic Acids Research 38:1~13
Gillman, L. M. / 2001 / Identification of Mycobacterium species by multiple-fluorescence PCR-single-strand conformation polymorphism analysis of the 16S rRNA gene / Journal of Clinical Microbiology 39:3085~3091
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