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논문 기본 정보

자료유형
학술저널
저자정보
Lee, Hong-Min (Food Safety Center, Research and Development Institute, Namyang Dariy Co., Ltd.) Kwak, Byung-Man (Food Safety Center, Research and Development Institute, Namyang Dariy Co., Ltd.) Ahn, Jang-Hyuk (Food Safety Center, Research and Development Institute, Namyang Dariy Co., Ltd.) Jeong, Seung-Hwan (Food Safety Center, Research and Development Institute, Namyang Dariy Co., Ltd.) Shim, Sung-Lye (Department of Food and Nutrition, Chosun University) Kim, Kyong-Su (Department of Food and Nutrition, Chosun University) Yoon, Tae-Hyung (Nutrition and Functional Food Research Team, Korea Food and Drug Administration) Leem, Dong-Gil (Nutrition and Functional Food Research Team, Korea Food and Drug Administration) Jeong, Ja-Young (Nutrition and Functional Food Research Team, Korea Food and Drug Administration)
저널정보
한국축산식품학회 한국축산식품학회지 한국축산식품학회지 제31권 제2호
발행연도
2011.1
수록면
191 - 199 (9page)

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The objective of this study was to develop a method to simultaneously quantify vitamins A and E in infant formula. To determine the vitamin A and E content, vitamin A and four different vitamin E isomers (${\alpha}$-, ${\beta}$-, ${\gamma}$-, and ${\delta}$-tocopherol) were separated by high performance liquid chromatography with a photodiode array detector using a Develosil RPAQUEOUS RP-$C_{30}$ column ($4.6{\times}250$ mm, 5 ${\mu}M$). The vitamin A and E contents in the certified reference material determined using this method were within the certified range of standard values. The limits of detection (LODs) and limits of quantitation (LOQs) for vitamin A were 0.02 and 0.06 ${\mu}g/L$, respectively. LODs and LOQs for the vitamin E isomers ranged from 0.20 to 0.55 and from 0.67 to 1.81 ${\mu}g/L$, respectively. Linear analyses indicated that the square of the correlation coefficient for the vitamin A and E isomers was 0.9997-0.9999. The recovery of vitamins ranged from 96.69 to 97.79%. The results demonstrate that this novel method could be used to reliably analyze vitamin A and E content in infant formula.

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