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논문 기본 정보

자료유형
학술저널
저자정보
Ahn, Jang-Hyuk (KOTITI Testing & Research Institute) Kwak, Byung-Man (Food Safety Center, Research and Development Institute, Namyang Dairy Co., Ltd.) Park, Jung-Min (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) Kim, Na-Kyeoung (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) Kim, Jin-Man (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
저널정보
한국축산식품학회 한국축산식품학회지 한국축산식품학회지 제34권 제6호
발행연도
2014.1
수록면
749 - 756 (8page)

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A rapid and simple analytical method for L-carnitine was developed for infant and toddler formulas by liquid chromatography tandem mass spectrometry (LC-MS/MS). A 0.3 g of infant formula and toddler formula sample was mixed in a 50 mL conical tube with 9 mL water and 1 mL 0.1 M hydrochloric acid (HCl) to chemical extraction. Then, chloroform was used for removing a lipid fraction. After centrifuged, L-carnitine was separated and quantified using LC-MS/MS with electrospray ionization (ESI) mode. The precursor ion for L-carnitine was m/z 162, and product ions were m/z 103 (quantitative) and m/z 85 (qualitative), respectively. The results for spiked recovery test were in the range of 93.18-95.64% and the result for certified reference material (SRM 1849a) was within the range of the certificated values. This method could be implemented in many laboratories that require time and labor saving.

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