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논문 기본 정보

자료유형
학술저널
저자정보
Ha, Woo Tae (Department of Animal & Food Bioscience, College of Natural Science, Konkuk University) Lee, Won Young (Department of Animal & Food Bioscience, College of Natural Science, Konkuk University) Lee, Ran (Department of Animal & Food Bioscience, College of Natural Science, Konkuk University) Kim, Jae Hwan (Departmemt of Biomedical Science, College of Life Science, CHA University) Kim, Nam Hyung (Department of Animal Science, College of Agriculture, Chungbuk National University) Kim, Jin Hoi (Department of Animal Biotechnology, Konkuk University) Lee, Il Joo (R&D Team, Darby Genetics Inc.) Song, Hyuk (Department of Animal & Food Bioscience, College of Natural Science, Konkuk University)
저널정보
한국동물번식학회 한국동물번식학회지 한국동물번식학회지 제37권 제1호
발행연도
2013.1
수록면
9 - 16 (8page)

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초록· 키워드

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Artificial insemination (AI) has been performed widely in swine industry using fresh liquid sperm instead of frozen type of sperm. However fresh sperm are not able to preserve more than three days with optimal motility and other sperm parameters for the successful fertilization, since in vitro stored sperm has an oxidative stress that resulted increase of abnormality and acrosome reation. To overcome these major problems, novel preservative formulation is needed to neutralize the oxidative stress and to provide suitable physiological environment for sperm in in vitro. In this study, naturally derived substances such as Poncirus trifoliate (Trifoliate orange), Garcinia mangostana (Mangosteen), pig placenta and testis extracts were tested as sperm preservative agents. Placenta extracts (PE), trifoliate orange extracts (TOE), testes extracts (TE) and mangosteen extracts (ME) were applied to analyze specific parameters for sperm motion characteristics individually and combinatorial. Each individual extract treatment can accelerate the sperm motility but noticeably TOE, TE and ME treatments exhibited the considerable and significant preservation of sperm motility. PE, TE and ME showed a significant (p<0.05) increase in ALH after one week. Further we evaluated the five different combinations of these extracts on sperm motility and its motion characteristics. Surprisingly even after one week ME, TOE and TE combination significantly preserved the sperm motility about 75%. It is noteworthy that unlike individual extract treatment, combination of ME, TOE and TE simultaneously protect the sperm motility and its motion characteristics. Taken together these data conclude that addition of ME, TOE and TE can be effective for preservation of pig sperm.

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