지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
Production of transgenic animals for studying specific gene has been limited due to a low efficiency, lack of skilled researchers and the need for expensive equipment. Currently, the boar spermatozoa as a vector to deliver exogenous DNA into the oocyte were used to improve the efficiency of transfection rate. In this study, we revealed that the optimal conditions for DNA uptake in spermatozoa by liposome were to 90 min of incubation, $17^{\circ}C$, $10^5$ spermatozoa, 4 ng/ml of exogenous DNA and 0.5% (v/v) liposome, without damage to fertility. In addition, the developmental rate to the blastocyst stage of embryo in control group was significantly higher than those embryos with exogenous DNA and liposome, whereas there were no significant differences in embryo development between the liposome and type of DNA. The transfection rates of embryo using treated spermatozoa with both liposome and circular DNA were higher than those using linear DNA. These findings raise the possibility thattreated spermatozoa with liposome/DNA complexes could be used in in vitro fertilization, and the exogenous DNA transferred into the oocytes. Taken together, we demonstrated that liposome a vector for the uptake of exogenous DNA in boar spermatozoa could improve the efficiency of sperm-mediated gene transfer in creating transgenic pig and the other domestic transgenic animals.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Uptake of exogenous DNA by using liposome in boar spermatozoa
한국수정란이식학회 심포지움
2005 .01
-
Uptake of Mitochondrial DNA fragment into Boar Spermatoza for Sperm-Mediated Gene Transfer
한국동물번식학회지
2006 .01
-
Positive Expression of EGFP in Bovine Embryos after ICSI using Spermatozoa Co-cultured with Exogenous DNA
한국동물번식학회 학술대회
2002 .01
-
In Vitro Embryo Development by Loaded Boar Spermatozoa with Exogenous DNA
한국수정란이식학회 심포지움
2006 .01
-
Transfer of Porcine Embryos Injected with Sperm Carrying with Exogenous DNA
한국동물번식학회 학술대회
2001 .01
-
Expression of GFP Gene in Porcine Embryos after ICSI with Different DNA Binding Methods
한국동물번식학회 학술대회
2004 .01
-
정소실질내 유전자 도입에 의한 형질저환동물의 생산 I. 형질전환 흰쥐와 생쥐의 생산
한국동물번식학회지
1998 .01
-
정소실질내 유전자 도입에 의한 형질전환동물의 생산 II. 형질전환 한국재래산양의 생산
한국동물번식학회지
1999 .01
-
외래유전자 도입정자를 이용한 돼지 체외성숙 난포란의 Intracytoplasmic Sperm Injection (ICSI) 후 후기 배로의 발달율과 외래유전자의 발현에 관한 연구
한국수정란이식학회지
2002 .01
-
Expression of GFP Gene in the Porcine Preimplantation Embryos after ICSI with DNA/Sperm Complex
한국동물번식학회지
2006 .01
-
Prolonged Expression of Exogenous GFP Gene in the Porcine Embryos generated by Intracytoplasmic Sperm Injection-Mediated Gene Transfer
한국수정란이식학회지
2015 .01
-
[P10-20] Antioxidant activities of phlorotannins purified from Ecklonia Cava on free radical scavenging using ESR and H2O2-mediated DNA damage
한국식품영양과학회 학술대회발표집
2006 .10
-
In Vitro Production of Pig Embryos using Intracytoplasmic Injection of Flow Cytometry Sorted Boar Spermatozoa
한국수정란이식학회지
2008 .01
-
The Short Time UV Laser Exposure do not cause DNA Damage in Boar Spermatozoa Assessed by Comet Assay
한국수정란이식학회 심포지움
2006 .01
-
Production of Transgenic Porcine Embryos by Oocyte Mediated Gene Transfer
한국수정란이식학회 심포지움
2006 .01
-
Efficiency of Transgenesis by Using Sperm Mediated Gene Transfer on the Cultured Prepubertal Mouse Testicular Cells
한국동물번식학회 학술대회
2004 .01
-
Effects of In Vitro Fertilization Conditions of In Vitro Matured Cumulus-Intact Pig Oocytes on Embryo Development
한국수정란이식학회지
2011 .01
-
활성산소계가 돼지 정자의 기능에 미치는 영향
한국동물번식학회지
2009 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0