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논문 기본 정보

자료유형
학술저널
저자정보
Won, Hyosig (Department of Biological Sciences, Daegu University) Yun, Young-Eun (National Institute of Biological Resources) Kwak, Myounghai (National Institute of Biological Resources) Han, Jeong Eun (National Institute of Biological Resources)
저널정보
국립생물자원관 Journal of species research Journal of species research 제1권 제2호
발행연도
2012.1
수록면
224 - 231 (8page)

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초록· 키워드

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To assess the genetic diversity of Aconitum coreanum (Ranunculaceae) populations in Korea, we have amplified and sequenced eight organellar marker regions, and developed and analyzed microsatellite markers. No sequence variation was detected from the eight organellar markers. Ten microsatellites were developed using Next Generation Sequencing and two microsatellite markers, AK_CA03 and AK_CT07, were identified polymorphic and applied for 143 individuals of twelve A. coreanum populations. Four and five alleles were detected for the two microsatellite loci, respectively, and number of migrants ($N_m$) was estimated as 1.12586. Two microsatellite marker loci showed $F_{ST}$ of 0.205 and 0.275, respectively. The heterozygosity deficit, low level of among-population differentiation, small size of gene flow, and lack of sequence variation of the organellar markers suggest that A. coreanum is reproductively isolated from other Aconitum species and there has been continuous gene flow among the populations of A. coreanum or it has dispersed relatively recently after speciation. Though population pairwise $F_{ST}$'s presented significant geographic structure, further sampling and study will be necessary to confirm this.

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