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논문 기본 정보

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학술저널
저자정보
Lee, Jong Hoon (Department of Plastic and Reconstructive Surgery, Eulji General Hospital, Eulji University College of Medicine) Lee, Kuk Han (Department of Plastic and Reconstructive Surgery, Eulji General Hospital, Eulji University College of Medicine) Kim, Min Ho (Eulji Medi-Bio Research Institute, Eulji University) Kim, Jun Pyo (CL Aesthetic Clinic) Lee, Seung Jae (CL Aesthetic Clinic) Yoon, Jinah (Department of Biotechnology, Seoul Women's University)
저널정보
대한성형외과학회 Archives of plastic surgery : APS Archives of plastic surgery : APS 제39권 제6호
발행연도
2012.1
수록면
593 - 599 (7page)

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Background This study aimed to investigate the possibility of isolating mesenchymal stem cells (MSCs) from human thigh adipose tissue and the ability of human thigh adipose stem cells (HTASCs) to differentiate into hepatocytes. Methods The adipose-derived stem cells (ADSCs) were isolated from thigh adipose tissue. Growth factors, cytokines, and hormones were added to the collagen coated dishes to induce the undifferentiated HTASCs to differentiate into hepatocyte-like cells. To confirm the experimental results, the expression of hepatocyte-specific markers on undifferentiated and differentiated HTASCs was analyzed using reverse transcription polymerase chain reaction and immunocytochemical staining. Differentiation efficiency was evaluated using functional tests such as periodic acid schiff (PAS) staining and detection of the albumin secretion level using enzyme-linked immunosorbent assay (ELISA). Results The majority of the undifferentiated HTASCs were changed into a more polygonal shape showing tight interactions between the cells. The differentiated HTASCs up-regulated mRNA of hepatocyte markers. Immunocytochemical analysis showed that they were intensely stained with anti-albumin antibody compared with undifferentiated HTASCs. PAS staining showed that HTASCs submitted to the hepatocyte differentiation protocol were able to more specifically store glycogen than undifferentiated HTASCs, displaying a purple color in the cytoplasm of the differentiated HTASCs. ELISA analyses showed that differentiated HTASCs could secrete albumin, which is one of the hepatocyte markers. Conclusions MSCs were islolated from human thigh adipose tissue differentiate to heapatocytes. The source of ADSCs is not only abundant abdominal adipose tissue, but also thigh adipose tissue for cell therapy in liver regeneration and tissue regeneration.
#Thigh #Adipose tissue #Stem cells #Hepatocytes #Cell Differentiation

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참고문헌 (19)

참고문헌 신청
Banas A / 2007 / Adipose tissue-derived mesenchymal stem cells as a source of human hepatocytes / Hepatology 46:219~228 google schola Talens-Visconti R / 2007 / Human mesenchymal stem cells from adipose tissue: differentiation into hepatic lineage / Toxicol In Vitro 21:324~329 google schola Planat-Benard V / 2004 / Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives / Circulation 109:656~663 google schola Seo MJ / 2005 / Differentiation of human adipose stromal cells into hepatic lineage in vitro and in vivo / Biochem Biophys Res Commun 328:258~264 google schola Oh SH / 2000 / Hepatocyte growth factor induces differentiation of adult rat bone marrow cells into a hepatocyte lineage in vitro / Biochem Biophys Res Commun 279:500~504 google schola

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