지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 363 - 366 (4page)
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초록· 키워드
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We recently demonstrated that non-plant cells, yeast WAT21, produce a steroidal plant hormone, castasterone. To understand how castasterone is generated in WAT21 cells, deuterium-labeled (26, $28-^2H_6$) 6-deoxoteasterone, 6-deoxotyphasterol and 6-deoxocastasterone were fed to WAT21 cells, and their metabolites were analyzed by gas chromatography-mass spectrometry. When [$^2H_6$]-6-deoxoteasterone was fed, [$^2H_6$]-6-deoxo-3-dehydroteasterone and [$^2H_6$]-6-deoxotyphasterol were identified as metabolites. When [$^2H_6$]-6-deoxotyphasterol was used, [$^2H_6$]-6-deoxoteasterone and [$^2H_6$]-6-deoxo-3-dehydroteasterone and [$^2H_6$]-6-deoxocastasterone were detected. When [$^2H_6$]-6-deoxocastasterone was added, [$^2H_6$]-castasterone was identified. Taken together, a biosynthetic sequence, 6-deoxoteasterone${\leftrightarrow}$6-deoxo-3-dehydroteasterone${\leftrightarrow}$6-deoxotyphasterol${\rightarrow}$6-deoxocastasterone${\rightarrow}$castasterone seems to function to produce brassinosteroids in WAT21 cells. Coupled with the presence of a biosynthetic sequence, teasterone${\rightarrow}$3-dehydroteasterone${\leftrightarrow}$typhasterol ${\rightarrow}$castasterone, this suggests that brassinosteroids in WAT21 cells were biosynthesized via the same kind of pathways established in plants. This study provides a clue for possible mass-production of brassinosteroids in non-plant cells, yeast WAT21 for commercial use in agriculture.
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