지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 417 - 422 (6page)
이용수
초록· 키워드
오류제보하기
The microbial transglutaminase (TGase) gene, stgA, was cloned from Streptomyces platensis YK-2, which was newly isolated from a forest soil sample collected in Daegu, Korea. The gene was expressed in several Streptomyces strains including the original strain as the host. The stgA consisted of an open reading frame of 1,257 bp encoding a putative signal peptide of 29 amino acids, a pro-domain of 57 amino acids, and a mature TGase of 332 amino acids, and also had the putative active site of TGase, YGCVG. For expression of the stgA gene, a high expression vector was constructed via the insertion of the entire stgA gene including its putative ribosomal binding site into pSET152ET, located immediately downstream of a strong constitutive $ermE^*$ promoter, and was introduced into several Streptomyces strains. The successful high expression and secretion of the correct StgA from streptomycetes were confirmed by electrophoretic sodium dodecyl sulfate polyacrylamide gel electrophoresis and N-terminal amino acid analysis.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청이 논문의 저자 정보
최근 본 자료
댓글(0)
0