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논문 기본 정보

자료유형
학술저널
저자정보
Lee, Sangshin (Department of Plastic and Reconstructive Surgery, Asan Medical Center, University of Ulsan College of Medicine) Jung, Inwook (Department of Plastic and Reconstructive Surgery, Asan Medical Center, University of Ulsan College of Medicine) Yu, Seongcheol (Department of Plastic Surgery, Gangneung Asan Hospital, University of Ulsan College of Medicine) Hong, Joon Pio (Department of Plastic and Reconstructive Surgery, Asan Medical Center, University of Ulsan College of Medicine)
저널정보
대한성형외과학회 Archives of plastic surgery : APS Archives of plastic surgery : APS 제41권 제5호
발행연도
2014.1
수록면
466 - 471 (6page)

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Background Bleeding can be a problem in wound debridement. In search for an effective hemostatic agent, we experimented with a chitosan film combined with the recombinant human epidermal growth factor (rh-EGF), hypothesizing that it would achieve effective hemostasis and simultaneously enhance arterial healing. Methods Forty-eight Sprague-Dawley rats were used, and 96 puncture wounds were made. The wounds were divided into the following four groups: treated with sterile gauze, treated with gelatin sponge, treated with chitosan, and treated with chitosan combined with rh-EGF. Immediate hemostasis was evaluated, and arterial healing was observed histologically. Results Groups B, C, and D showed a significant rate of immediate hemostasis as compared to group A (P<0.05), but there were no significant differences among groups B, C, and D. Histologically, only group D showed good continuity of the vessel wall after 1 week. It was the only group to show smooth muscle cell nuclei of the vessel wall. Conclusions We observed that chitosan has an effective hemostatic potential and the mix of rh-EGF and chitosan does not interfere with chitosan's hemostatic capabilities. We also identified enhanced healing of vessel walls when rh-EGF was added to chitosan. Further research based on these positive findings is needed to evaluate the potential use of this combination on difficult wounds like chronic diabetic ulcerations.

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