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논문 기본 정보

자료유형
학술저널
저자정보
Bankeeree, Wichanee (Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University) Lotrakul, Pongtharin (Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University) Prasongsuk, Sehanat (Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University) Kim, Seung Wook (Department of Chemical and Biological Engineering, Korea University) Punnapayak, Hunsa (Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University)
저널정보
한국화학공학회 화학공학 화학공학 제54권 제6호
발행연도
2016.1
수록면
822 - 829 (8page)

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Our aim was to optimize the production of cellulase-free thermoactive xylanase by Aureobasidium pullulans CBS 135684 with statistical methodology based on experimental designs. Among eleven variables, the nutrient sources that had significant effect on xylanase production were corncob, $(NH_4)_2SO_4$, xylose, $KH_2PO_4$ and tween 80, identified by the initial screening method of Plackett-Burman. The optimum concentrations of these five components were subsequently investigated using response surface methodology. The optimal concentrations ($g{\cdot}l^{-1}$) for maximum production of xylanase were corncob, 39.0; $(NH_4)_2SO_4$, 3.0; xylose, 1.8; $KH_2PO_4$ 1.4; and tween 80, 1.4, respectively. An improved xylanase yield of $8.74{\pm}0.84U{\cdot}ml^{-1}$ was obtained with optimized medium which is 2.1-fold higher production than previously obtained results ($4.10{\pm}0.10U{\cdot}ml^{-1}$) after 48 h of cultivation. In addition, the xylanase production under optimal condition reached $10.09{\pm}0.27U{\cdot}ml^{-1}$ after 72 h of cultivation.

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