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학술저널
저자정보
Lee, Jae-Jung (Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research) Kim, Jun-Young (Department of Chemistry, MedChem Program of Life Sciences Institute, National University of Singapore) Zhai, Duanting (Department of Chemistry, MedChem Program of Life Sciences Institute, National University of Singapore) Yun, Seong-Wook (Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research) Chang, Young-Tae (Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research)
저널정보
한국생물정보시스템생물학회 Interdisciplinary Bio Central Interdisciplinary Bio Central 제4권 제2호
발행연도
2012.1
수록면
51 - 59 (9page)

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Small peptide tags such as c-myc, HA, or FLAG tag have facilitated efficient Western-blotting of proteins of interest especially when specific antibodies for the proteins are not available. However, the conventional Western-blotting requires the multi-steps process taking at least several hours up to two days. With examples of various applications, here we show a convenient and time-saving method for protein detection which employs a fluorescent chemical BDED and its binding peptide RC-tag. And we propose "Estern staining", as a standard term for protein detection method using fluorescent chemicals and their binding small peptide tags. Eastern staining may substitutes for the time-consuming "immuno-staining" in many versatile applications.

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