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자료유형
학술저널
저자정보
Kim, Hak-Jae (College of Medicine, Soonchunhyang University) Kang, Sung-Wook (Kohwang Medical Research Institute, School of Medicine, Kyung Hee University) Kim, Mi-Ja (Kohwang Medical Research Institute, School of Medicine, Kyung Hee University) Kim, Young-Ock (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration College of Medicine, Soonchunhyang University) Kim, Geum-Soog (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration College of Medicine, Soonchunhyang University) Park, Yoon-Hyung (College of Medicine, Soonchunhyang University) Chung, Joo-Ho (Kohwang Medical Research Institute, School of Medicine, Kyung Hee University)
저널정보
대한독성유전단백체학회 Molecular & cellular toxicology Molecular & cellular toxicology 제6권 제3호
발행연도
2010.1
수록면
219 - 227 (9page)

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To investigate the effects of Panax ginseng (PG) on SH-SY5Y human neuroblastoma cells, we profiled gene expressions of both normal and PG-treated groups using cDNA microarray. We performed 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays to determine the effect on PG-induced cytotoxicity. The gene expression profiles in normal group were compared to PG-treated group using 24K human cDNA microarray. MTT assay showed that PG was not cytotoxic at the concentrations of $1-100\;{\mu}g/mL$ in SH-SY5Y cells. Consequently, 210 genes exhibited differential expression levels between normal and PG-treated groups through cDNA microarray analysis, and it was found that 52 of the 210 genes were up-regulated while 146 of them were down-regulated by two-fold. PG may induce changes in the expression of genes in SH-SY5Y cells possibly relevant to signal transduction and cellular development. These results help to further understand the molecular framework of PG as a signal transduction inducer of neuronal cells.

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