지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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A novel lectin specific to N-acetyl-D-galactosamine as well as N-acetyl-D-glucosamine was isolated from Bryopsis plumosa and named as BPL-4. Sodium dodecyl sulfate polyacrylamide gel electrophorese (SDS-PAGE) and matrix-assisted laser desorption / ionization-time of flight (MALDI-TOF) mass spectrometry data showed that this lectin was a monomeric protein with molecular weight 12.9 kDa. The N-terminal amino acid sequences of the lectin were determined by Edman degradation and the full cDNA sequence encoding this lectin was obtained using the degenerate primers designed from the amino acid sequence. The size of the cDNA was 414 bp containing single open reading frame (ORF) encoding the lectin precursor. The homology analysis showed that this lectin might belong to H lectin group. BPL-4 showed high sequence similarity (60.6%) to BPL-3, which is a previously reported lectin from the same species. The comparative analysis on the lectin's primary structure showed two conserved domains including one possible active domain of H lectin group.
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참고문헌 (25)
참고문헌 신청
Bogoeva, V. P. / 2008 / Fluorescence study of steroid hormone binding activity of Helix pomatia agglutinin / Steroids 73:1060~1065
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72:248~254
Goldstein, I. J. / 1980 / What should be called a lectin? / Nature 285:66
한종원 / 201004 / Purification and characterization of a D-mannose specific lectin from the green marine alga, Bryopsis plumosa / PHYCOLOGICAL RESEARCH 58:143~150
한종원 / 201108 / Purification and characterization of a lectin, BPL-3, from the marine green alga Bryopsis plumosa / JOURNAL OF APPLIED PHYCOLOGY 23(4):745~753
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72:248~254
Goldstein, I. J. / 1980 / What should be called a lectin? / Nature 285:66
한종원 / 201004 / Purification and characterization of a D-mannose specific lectin from the green marine alga, Bryopsis plumosa / PHYCOLOGICAL RESEARCH 58:143~150
한종원 / 201108 / Purification and characterization of a lectin, BPL-3, from the marine green alga Bryopsis plumosa / JOURNAL OF APPLIED PHYCOLOGY 23(4):745~753
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