지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Protein profiles of a common mixotrophic dinoflagellate, Prorocentrum micans, growing autotrophically and mixotrophically (fed on the cryptophyte Rhodomonas salina) were compared using two-dimensional gel electrophoresis (2-DE) to determine if they vary in different trophic modes. Approximately 2.3% of the detected proteins were differentially expressed in the different trophic modes. Twelve proteins observed only in the mixotrophic condition had lower pI value (<5) than the fifteen proteins observed only in the autotrophic condition (>5). When the internal amino acid sequences of five selected proteins differentially expressed between autotrophic and mixotrophic conditions were analyzed using matrix-assisted laser desorption time-of-flight (MALDI-TOF) mass spectrometry, two proteins that were specifically expressed in the autotrophic condition showed homology to glyceraldehyde-3-phosphatase dehydrogenase (GAPDH) and a bacterial catalase. Three mixotrophy-specific proteins showed homology to certain hypothetical proteins from an insect and bacteria. These results suggested the presence of certain gene groups that are switched on and off according to the trophic mode of P. micans.
목차
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참고문헌 (26)
참고문헌 신청
Akimoto, H. / 2004 / Biological rhythmicity in expressed proteins of the marine dinoflagellate Lingulodinium polyedrum demonstrated by chronological proteomics / Biochem. Biophys. Res. Commun 315:306~312
Bockstahler, K. R. / 1993 / Grazing of the mixotrophic dinoflagellate Gymnodinium sanguineum on ciliate populations of Chesapeake Bay / Mar. Biol 116:447~487
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72:248~254
Burkholder, J. M. / 2008 / Mixotrophy, a major mode of nutrition for harmful algal species in eutrophic waters / Harmful Algae 8:77~93
Cerff, R. / 1995 / The chimeric nature of nuclear genomes and the antiquity of introns as demonstrated by GAPDH gene system. in Tracing Biological Evolution in Protein and Gene Structures / Elsevier :205~227
Bockstahler, K. R. / 1993 / Grazing of the mixotrophic dinoflagellate Gymnodinium sanguineum on ciliate populations of Chesapeake Bay / Mar. Biol 116:447~487
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72:248~254
Burkholder, J. M. / 2008 / Mixotrophy, a major mode of nutrition for harmful algal species in eutrophic waters / Harmful Algae 8:77~93
Cerff, R. / 1995 / The chimeric nature of nuclear genomes and the antiquity of introns as demonstrated by GAPDH gene system. in Tracing Biological Evolution in Protein and Gene Structures / Elsevier :205~227
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