지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Polysaccharides prepared from marine algae sometimes contain contaminants such as polyphenols and endotoxins that may mislead their bona fide biological activities. In this study, we examined bioactive contaminants in commercially available fucoindan from Fucus vesiculosus, along with ascophyllan and fucoidan from Ascophyllum nodosum. F. vesiculosus fucoidan inhibited the growth of Vibrio alginolyticus in a concentration-dependent manner ($0-1,000{\mu}g\;mL^{-1}$). However, the antibacterial activity of the fucoidan significantly reduced after methanol-extraction, and the methanol-extract showed a potent antibacterial activity. The extract also showed cytotoxicity to RAW264.7 and U937 cells, and induced apoptotic nuclear morphological changes in U937 cells. These results suggest that the antibacterial activity of the fucoidan is partly due to the methanol-extractable contaminants that can also contribute to the cytotoxicity on RAW264.7 and U937 cells. On the other hand, the activities to induce secretion of nitric oxide and tumor necrosis factor-${\alpha}$ from RAW264.7 cells were observed in the fucoidan even after methanol extraction, and the extract had no such activities. Our observations suggest that commercially available fucoidan should be purified prior to biochemical use.
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참고문헌 (40)
참고문헌 신청
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Baba, M. / 1990 / Sulfated polysaccharides as potent inhibitors of HIV-induced syncytium formation: a new strategy towards AIDS chemotherapy / J. Acquir. Immune Defic. Syndr 3:493~499
Chotigeat, W. / 2004 / Effect of fucoidan on disease resistance of black tiger shrimp / Aquaculture 233:23~30
Clément, M. J. / 2010 / NMR characterization and molecular modeling of fucoidan showing the importance of oligosaccharide branching in its anticomplementary activity / Glycobiology 20:883~894
Collins, L. / 1997 / Microplate alamar blue assay versus BACTEC 460 system for high-throughput screening of compounds against Mycobacterium tuberculosis and Mycobacterium avium / Antimicrob. Agents Chemother 41:1004~1009
Baba, M. / 1990 / Sulfated polysaccharides as potent inhibitors of HIV-induced syncytium formation: a new strategy towards AIDS chemotherapy / J. Acquir. Immune Defic. Syndr 3:493~499
Chotigeat, W. / 2004 / Effect of fucoidan on disease resistance of black tiger shrimp / Aquaculture 233:23~30
Clément, M. J. / 2010 / NMR characterization and molecular modeling of fucoidan showing the importance of oligosaccharide branching in its anticomplementary activity / Glycobiology 20:883~894
Collins, L. / 1997 / Microplate alamar blue assay versus BACTEC 460 system for high-throughput screening of compounds against Mycobacterium tuberculosis and Mycobacterium avium / Antimicrob. Agents Chemother 41:1004~1009
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