지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Intact phycobilisomes from a wild-type red Chondrus crispus and its vegetatively derived green mutant were isolated by centrifugation through a discontinuous sucrose density gradient. Pigment composition was subsequently characterized by spectrophotometry. Vegetative thalli of the two strains grown together for six months in the laboratory resulted in different pigment profiles. Two pigmented phycobilisome bands appeared in the sucrose gradient of the wild-type alga, a purple coloured one, and a pink one, whereas only a single blue band appeared in the gradient of the green mutant. Spectrophotometric and fluorescence analyses identified the phycobiliprotein composition of the purple band as the typical phycoerythrin-phycocyanin-allophycocyanin complement in the wild-type, but there was no detectable phycoerythrin present in the blue band of the green mutant. Sodium dodecyl sulphate, preparative polyacrylamide gel electrophoresis analysis confirmed the presence of allophycocyanin subunits in all extracts, but firm evidence of an R-phycoerythrin linker polypeptide in the blue band was missing. These results highlight the ability of C. crispus to adapt to a phycoerythrin deficiency by adjusting light harvesting pigment ratios.
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참고문헌 (39)
참고문헌 신청
Algarra, P. / 1990 / Phycobilisome heterogeneity in the red alga Porphyra umbilicalis / Plant Physiol 92:570~576
Arteni, A. A. / 2008 / Structure and organization of phycobilisomes on membranes of the red alga Porphyridium cruentum / Photosynth. Res 95:169~174
Beer, S. / 1985 / Determining phycoerythrin and phycocyanin concentrations in aqueous crude extracts of red algae / Aust. J. Mar. Freshw. Res 36:785~792
Bozzola, J. J. / 1999 / Electron microscopy / Jones and Bartlett Publishers :655
Costa, V. L. / 2011 / Color inheritance and pigment characterization of red (wild-type), greenishbrown, and green strains of Gracilaria birdiae (Gracilariales, Rhodophyta) / J. Appl. Phycol 23:599~605
Arteni, A. A. / 2008 / Structure and organization of phycobilisomes on membranes of the red alga Porphyridium cruentum / Photosynth. Res 95:169~174
Beer, S. / 1985 / Determining phycoerythrin and phycocyanin concentrations in aqueous crude extracts of red algae / Aust. J. Mar. Freshw. Res 36:785~792
Bozzola, J. J. / 1999 / Electron microscopy / Jones and Bartlett Publishers :655
Costa, V. L. / 2011 / Color inheritance and pigment characterization of red (wild-type), greenishbrown, and green strains of Gracilaria birdiae (Gracilariales, Rhodophyta) / J. Appl. Phycol 23:599~605
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