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논문 기본 정보

자료유형
학술저널
저자정보
Hong, Ji-Won (Department of Biology, Kyungpook National University) Choi, Han-Gu (Division of Polar Biology & Ocean Sciences, Korea Polar Research Institute [KOPRI]) Kang, Sung-Ho (Division of Polar Biology & Ocean Sciences, Korea Polar Research Institute [KOPRI]) Yoon, Ho-Sung (Department of Biology, Kyungpook National University)
저널정보
한국조류학회(藻類) Algae Algae 제25권 제2호
발행연도
2010.1
수록면
99 - 104 (6page)

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초록· 키워드

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A psychrotolerant cyanobacterium, Nodularia spumigena KNUA005, was isolated from a cyanobacterial bloom sample collected near Dasan Station in Ny-${\AA}lesund$, Svalbard Islands during the Arctic summer season. To generate an axenic culture, the isolate was subjected to three purification steps: centrifugation, antibiotic treatment and streaking. The broad antibacterial spectrum of imipenem killed a wide range of heterotrophic bacteria, while the cyanobacterium was capable of enduring both antibiotics, the remaining contaminants that survived after treatment with imipenem were eliminated by the application of an aminoglycoside antibiotic, kanamycin. Physical separation by centrifugation and streaking techniques also aided axenic culture production. According to the cold-tolerance test, this mat-forming cyanobacterium was able to proliferate at low temperatures ranging between 15 and $20^{\circ}C$ which indicates the presence of cold-tolerance related genes in N. spumigena KNUA005. This suggests the possibility of incorporating cold-resistance genes into indigenous cyanobacterial strains for the consistent production of algae-based biofuel during the low-temperature seasons. Therefore, it is needed to determine the cold-tolerance mechanisms in the Arctic cyanobacterium in the next research stage.

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