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논문 기본 정보

자료유형
학술저널
저자정보
Choi, Sun-Gyu (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) Jang, Kab-Yeul (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) Kim, Gyu-Hyun (Dep. of Biotechnology, Cheonan Yonam College) Kong, Won-Sik (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) Jo, Jae-Sun (Dep. of Food Science and Biotechnology, Kyung Hee University) Kim, Hae-Yeong (Dep. of Food Science and Biotechnology, Kyung Hee University) Yoo, Young-Bok (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA)
저널정보
한국버섯학회 한국버섯학회지 한국버섯학회지 제12권 제3호
발행연도
2014.1
수록면
145 - 153 (9page)

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Pleurotus has increased rapidly production and consumption because of highly nutritional value, natural healthy food and so on. The basic studies for Pleurotus need for development of mushroom industry. This study was to investigate the cultural characteristics among 15 strains of 6 species and to analyze their phylogenetic relationships. The cultural characteristics were investigated by mycelial growth activity at different media, temperature and pH. The optimum media for mycelial growth were YM and MCM in most species. The optimum temperature for mycelial growth were $25^{\circ}C$ and $30^{\circ}C$. The optimum pH for mycelial growth were widly range from pH 5.1 to 7.4. Through the RFLP (restriction fragment length polymorphism) of IGS (intergenic spacer) I region in ribosomal DNA, it was analyzed phylogeny of interspecies and intraspecies. Each species was discriminated well as isolates within each species formed clade to be distinguished other species. P. florida was highly similar to P. floridanus, and P. flabellatus was P. cornucopiae. P. fuscus var. ferulae was highly similar to P. eryngii but discriminated different species in analysis of RFLP of IGS I region and showed different characteristics in mycelial culture. RFLP of IGS I region was useful of studying phylogenetic relationships of species and population.

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