Peroxidation of lipids in liposomal membranes of different composition photosensitized by chlorpromazine

Wolnicka-Glubisz, Agnieszka; Lukasik, Monika; Pawlak, Anna; Wielgus, Albert; Niziolek-Kierecka, Magda; Sarna, Tadeusz

추천

검색

자료유형: 학술저널

저자정보: Wolnicka-Glubisz, Agnieszka (Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University) Lukasik, Monika (Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University) Pawlak, Anna (Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University) Wielgus, Albert (Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University) Niziolek-Kierecka, Magda (Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University) Sarna, Tadeusz (Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University)

저널정보: 한국광과학회 Photochemical & photobiological sciences : an international journal Photochemical & photobiological sciences : an international journal 제8권 제2호

발행연도: 2009.1

수록면: 241 - 247 (7page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Chlorpromazine (CPZ), a phenothiazine derivative, is a neuroleptic drug widely used in medicine because of its tranquilizing and antipsychotic properties. CPZ often causes side effects including cutaneous photosensitization and ocular damage. It was suggested that reactive oxygen species, including singlet oxygen, might play an important role in its phototoxicity. In this work, we studied effects of cholesterol and saturation of fatty acid phospholipids on peroxidation of liposomal lipids induced by UVA irradiation in the presence of chlorpromazine by measuring lipid hydroperoxides using HPLC-EC(Hg) and the iodometric assay. Our study shows that the CPZ-photosensitized peroxidation of lipids is modified by cholesterol and the type of phospholipids present in the liposomes. Thus in liposomes made of the unsaturated 1-palmitoyl-2-oleoyl-phosphatidylcholine (POPC), chlorpromazine photosensitized strong peroxidation of lipids, which was mainly due to generation of singlet oygen. In such liposomes, CPZ-photosensitized peroxidation of lipids was decreased, in a dose-dependent manner, by addition of cholesterol. On the other hand, in liposomes made of dimyristoylphosphatidylcholine (DMPC) and cholesterol peroxidation of lipids was 5 fold slower, while generation of singlet oxygen was reduced over 20 fold. Importantly, cholesterol had no effect on lipid peroxidation in POPC and DMPC liposomes induced by other photosensitizers such as Rose Bengal and merocyanine 540. We postulate that the effect of cholesterol on peroxidation of lipids in liposomal membranes, photosensitized by chlorpromazine, is mostly due to the cholesterol-dependent modulation of CPZ partition in such membranes, which dramatically lowers the efficiency of the photoexcited CPZ to generate singlet oxygen.

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!