In Vitro and In Vivo Evaluation of Composite Scaffold of BCP, Bioglass and Gelatin for Bone Tissue Engineering

Kim, Woo Seok; Nath, Subrata Deb; Bae, Jun Sang; Padalhin, Andrew; Kim, Boram; Song, Myeong Jin; Min, Young Ki

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자료유형: 학술저널

저자정보: Kim, Woo Seok (Department of Physiology, Soonchunhyang University) Nath, Subrata Deb (Institute of Tissue Regeneration, Soonchunhyang University) Bae, Jun Sang (Institute of Tissue Regeneration, Soonchunhyang University) Padalhin, Andrew (Department of Regenerative Medicine, School of Medicine, Soonchunhyang University) Kim, Boram (Department of Regenerative Medicine, School of Medicine, Soonchunhyang University) Song, Myeong Jin (Department of Regenerative Medicine, School of Medicine, Soonchunhyang University) Min, Young Ki (Department of Physiology, Soonchunhyang University)

저널정보: 한국재료학회 한국재료학회지 한국재료학회지 제24권 제6호

발행연도: 2014.1

수록면: 310 - 318 (9page)

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In this experiment, a highly porous scaffold of biphasic calcium phosphate (BCP) was prepared using the spongereplica method. The BCP scaffold was coated with 58S bioactive glass (BG) and sintered for a second time. The resulting scaffold was coated with gelatin (Gel) and cross-linked with [3-(3-dimethyl aminopropyl) carbodiimide] and N-Hydroxysuccinamide (EDC-NHS). The initial average pore size of the scaffold ranged from 300 to $700{\mu}m$, with more than 85 % porosity. The coating of BG and Gel had a significant effect on the scaffold-pore size, decreasing scaffold porosity while increasing mechanical strength. The material and surface properties were evaluated by means of several experiments involving scanning electron microscopy (SEM), energy-dispersive X-ray (EDX) and X-ray diffraction (XRD). Cytotoxicity was evaluated using MTT assay and confocal imaging of MC3T3-E1 pre-osteoblast cells cultured in vitro. Three types of scaffold (BCP, BCP-BG and BCP-BG-Gel) were implanted in a rat skull for in vivo evaluation. After 8 weeks of implantation, bone regeneration occurred in all three types of sample. Interestingly, regeneration was found to be greater (geometrically and physiologically) for neat BCP scaffolds than for two other kinds of composite scaffolds. However, the other two types of scaffolds were still better than the control (i.e., defect without treatment).

#sponge replica #BCP #bioglass #gelatin

참고문헌 (27)

참고문헌 신청

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