Regulatory Effects of WRAP53 on Radiosensitivity of Laryngeal Squamous Cell Carcinoma Cells

Qiu, Hui; Zhao, De-Ying; Yuan, Li-Mei; Zhang, Gong; Xie, Cong-Hua

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자료유형: 학술저널

저자정보: Qiu, Hui (Department of Radiation & Medical Oncology, Zhongnan Hospital of Wuhan University, Cancer Clinical Study Center of Hubei Provinces, Key Laboratory of Tumor Biological Behavior of Hubei Pr) Zhao, De-Ying (Department of Radiation & Medical Oncology, Zhongnan Hospital of Wuhan University, Cancer Clinical Study Center of Hubei Provinces, Key Laboratory of Tumor Biological Behavior of Hub) Yuan, Li-Mei (Department of Radiation & Medical Oncology, Zhongnan Hospital of Wuhan University, Cancer Clinical Study Center of Hubei Provinces, Key Laboratory of Tumor Biological Behavior of Hube) Zhang, Gong (Department of Radiation & Medical Oncology, Zhongnan Hospital of Wuhan University, Cancer Clinical Study Center of Hubei Provinces, Key Laboratory of Tumor Biological Behavior of Hubei) Xie, Cong-Hua (Department of Radiation & Medical Oncology, Zhongnan Hospital of Wuhan University, Cancer Clinical Study Center of Hubei Provinces, Key Laboratory of Tumor Biological Behavior of Hub)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제16권 제7호

발행연도: 2015.1

수록면: 2,975 - 2,979 (5page)

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Background: Telomere length is closely associated with cellular radiosensitivity and WRAP53 is required for telomere addition by telomerase. In this research we assessed radiosensitivity of laryngeal squamous cell carcinoma Hep-2 cell lines after WRAP53 inhibition, and analyzed the molecular mechanisms. Materials and Methods: phWRAP53-siRNA and pNeg-siRNA were constructed and transfected into Hep-2 cells with lipofectamine. Expression of WRAP53 was analyzed by RT-PCR and Western-blottin, radiosensitivity of Hep-2 cells was assessed colony formation assay, and the relative length of telomeres was measured by QPCR. Results: The data revealed that the plasmid of phWRAP53-siRNA was constructed successfully, and the mRNA and protein levels of WRAP53 were both obviously reduced in the Hep-2 cell line transfected with phWRAP53-siRNA. After Hep-2 cells were irradiated with X-rays, the $D_0$ and $SF_2$ were 2.481 and 0.472, respectively, in the phWRAP53-siRNA group, much lower than in the control group ($D_0$ and $SF_2$ of 3.213 and 0.592) (P<0.01). The relative telomere length in the phWRAP53-siRNA group was $0.185{\pm}0.01$, much lower than in the untreated group ($0.523{\pm}0.06$) and the control group ($0.435{\pm}0.01$). Conclusions: Decreasing the expression of WRAP53 using RNA interference technique can enhance the radiosensitivity of Hep-2 cell lines by influencing the telomere length. WRAP53 is expected to be a new target to regulate the radiosensitization of tumor cells.

#WRAP53 #Hep-2 #laryngeal squamous cell carcinoma #radiosensitivity #telomere length

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Qiu, Hui

소속기관 Department of Radiation & Medical Oncology, Zhongnan Hospital of Wuhan University, Cancer Clinic