Identifying Differentially Expressed Genes and Screening Small Molecule Drugs for Lapatinib-resistance of Breast Cancer by a Bioinformatics Strategy

Zhuo, Wen-Lei; Zhang, Liang; Xie, Qi-Chao; Zhu, Bo; Chen, Zheng-Tang

추천

검색

자료유형: 학술저널

저자정보: Zhuo, Wen-Lei (Institute of Cancer, Xinqiao Hospital, Third Military Medical University) Zhang, Liang (Institute of Cancer, Xinqiao Hospital, Third Military Medical University) Xie, Qi-Chao (Institute of Cancer, Xinqiao Hospital, Third Military Medical University) Zhu, Bo (Institute of Cancer, Xinqiao Hospital, Third Military Medical University) Chen, Zheng-Tang (Institute of Cancer, Xinqiao Hospital, Third Military Medical University)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제15권 제24호

발행연도: 2014.1

수록면: 10,847 - 10,853 (7page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Background: Lapatinib, a dual tyrosine kinase inhibitor that interrupts the epidermal growth factor receptor (EGFR) and HER2/neu pathways, has been indicated to have significant efficacy in treating HER2-positive breast cancer. However, acquired drug resistance has become a very serious clinical problem that hampers the use of this agent. In this study, we aimed to screen small molecule drugs that might reverse lapatinib-resistance of breast cancer by exploring differentially expressed genes (DEGs) via a bioinformatics method. Materials and Methods: We downloaded the gene expression profile of BT474-J4 (acquired lapatinib-resistant) and BT474 (lapatinib-sensitive) cell lines from the Gene Expression Omnibus (GEO) database and selected differentially expressed genes (DEGs) using dChip software. Then, gene ontology and pathway enrichment analyses were performed with the DAVID database. Finally, a connectivity map was utilized for predicting potential chemicals that reverse lapatinib-resistance. Results: A total of 1, 657 DEGs were obtained. These DEGs were enriched in 10 pathways, including cell cycling, regulation of actin cytoskeleton and focal adhesion associate examples. In addition, several small molecules were screened as the potential therapeutic agents capable of overcoming lapatinib-resistance. Conclusions: The results of our analysis provided a novel strategy for investigating the mechanism of lapatinib-resistance and identifying potential small molecule drugs for breast cancer treatment.

#Lapatinib-resistance #differentially expressed genes #dysfunctional pathway #breast cancer #bioinformatics

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요