Trichostatin A-induced Apoptosis is Mediated by Kruppel-like Factor 4 in Ovarian and Lung Cancer

Zohre, Sadeghi; Kazem, Nejati-Koshki; Abolfazl, Akbarzadeh; Mohammad, Rahmati-Yamchi; Aliakbar, Movassaghpour; Effat, Alizadeh; Zahra, Davoudi; Hassan, Dariushnejad; Nosratollah, Zarghami

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자료유형: 학술저널

저자정보: Zohre, Sadeghi (Hematology and Oncology Research Center) Kazem, Nejati-Koshki (Department of Medical Biotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences) Abolfazl, Akbarzadeh (Department of Medical Nanotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences) Mohammad, Rahmati-Yamchi (Hematology and Oncology Research Center) Aliakbar, Movassaghpour (Department of Hematology, Faculty of Medicine, Tabriz University of Medical Sciences) Effat, Alizadeh (Department of Medical Biotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences) Zahra, Davoudi (Department of Medical Biotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences) Hassan, Dariushnejad (Department of Medical Biotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences) Nosratollah, Zarghami (Hematology and Oncology Research Center)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제15권 제16호

발행연도: 2014.1

수록면: 6,581 - 6,586 (6page)

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Background: The istone deacetylase (HDAC) inhibitor trichostatin A (TSA) is known to mediate the regulation of gene expression and antiproliferation activity in cancer cells. Kr$\ddot{u}$ppel-like factor 4 (klf4) is a zinc finger-containing transcription factor of the SP/KLF family, that is expressed in a variety of tissues and regulates cell proliferation, differentiation, tumorigenesis, and apoptosis. It may either either function as a tumor suppressor or an oncogene depending on genetic context of tumors. Aims: In this study, we tested the possibility that TSA may increase klf4 expression and cancer cell growth inhibition and apoptosis in SKOV-3 and A549 cells. Materials and Methods: The cytotoxicity of TSA was determined using the MTT assay test, while klf4 gene expression was assessed by real time PCR andto ability of TSA to induce apoptosis using a Vybrant Apoptosis Assay kit. Results: Our results showed that TSA exerted dose and time dependent cytotoxicity effect on SKOV-3 and A549 cells. Moreover TSA up-regulated klf4 expression. Flow cytometric analysis demonstrated that apoptosis was increased after TSA treatment. Conclusions: Taken together, this study showed that TSA increased klf4 expression in SKOV3 and A549 cell lines, consequently, klf4 may played a tumor-suppressor role by increasing both cell growth inhibition and apoptosis. This study sheds light on the details of molecular mechanisms of HDACI-induced cell cycle arrest and apoptosis.

#Histone deacetylase inhibitor #Trichostatin A #klf4 #apoptosis

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