Cytotoxic T Lymphocytes Elicited by Dendritic Cell-Targeted Delivery of Human Papillomavirus Type-16 E6/E7 Fusion Gene Exert Lethal Effects on CaSki Cells

Wu, Xiang-Mei; Liu, Xing; Jiao, Qing-Fang; Fu, Shao-Yue; Bu, You-Quan; Song, Fang-Zhou; Yi, Fa-Ping

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자료유형: 학술저널

저자정보: Wu, Xiang-Mei (Chongqing Molecular Medicine and Cancer Research Center, Chongqing Children's Hospital) Liu, Xing (Department of Pediatric Urology, Chongqing Children's Hospital) Jiao, Qing-Fang (Chongqing Molecular Medicine and Cancer Research Center, Chongqing Children's Hospital) Fu, Shao-Yue (Chongqing Molecular Medicine and Cancer Research Center, Chongqing Children's Hospital) Bu, You-Quan (Chongqing Molecular Medicine and Cancer Research Center, Chongqing Children's Hospital) Song, Fang-Zhou (Chongqing Molecular Medicine and Cancer Research Center, Chongqing Children's Hospital) Yi, Fa-Ping (Chongqing Molecular Medicine and Cancer Research Center, Chongqing Children's Hospital)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제15권 제6호

발행연도: 2014.1

수록면: 2,447 - 2,451 (5page)

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Human papillomavirus (HPV) is the primary etiologic agent of cervical cancer. Consideration of safety and non human leukocyte antigen restriction, protein vaccine has become the most likely form of HPV therapeutic vaccine, although none have so far been reported as effective. Since tumor cells consistently express the two proteins E6 and E7, most therapeutic vaccines target one or both of them. In this study, we fabricated DC vaccines by transducing replication-defective recombinant adenoviruses expressing E6/E7 fusion gene of HPV-16, to investigate the lethal effects of specific cytotoxic T lymphocytes (CTL) against CaSki cells in vitro. Mouse immature dendritic cells (DC) were generated from bone marrow, and transfected with pAd-E6/E7 to prepare a DC vaccine and to induce specific CTL. The surface expression of CD40, CD68, MHC II and CD11c was assessed by flow cytometry (FCM), and the lethal effects of CTL against CaSki cells were determined by DAPI, FCM and CCK-8 methods. Immature mouse DC was successfully transfected by pAd-E6/E7 in vitro, and the transfecting efficiency was 40%-50%. A DC vaccine was successfully prepared and was used to induce specific CTL. Experimental results showed that the percentage of apoptosis and killing rate of CaSki cells were significantly increased by coculturing with the specific CTL (p <0.05). These results illustrated that a DC vaccine modified by HPV-16 E6/E7 gene can induce apoptosis of CaSki cells by inducing CTL, which may be used as a new strategy for biological treatment of cervical cancer.

#Cervical cancer #Human papillomavirus #E6/E7 protein #CaSki cell

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