Macrophages Promote Coal Tar Pitch Extract-induced Tumorigenesis of BEAS-2B Cells and Tumor Metastasis in Nude Mice Mediated by AP-1

Zhang, Peng; Jin, Yue-Fei; Zhang, Qiao; Wu, Yi-Ming; Wu, Wei-Dong; Yao, Wu; Wu, Yong-Jun; Li, Zhi-Tao; Zhao, Yong; Liu, Yu; Feng, Fei-Fei

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자료유형: 학술저널

저자정보: Zhang, Peng (Department of Bone and Soft Tissue Cancer, The Affiliated Cancer Hospital of Zhengzhou University [Henan Cancer Hospital]) Jin, Yue-Fei (Department of Occupational and Environmental Health, College of Public Health, Zhengzhou University) Zhang, Qiao (Department of Toxicology, College of Public Health, Zhengzhou University) Wu, Yi-Ming (Department of Occupational and Environmental Health, College of Public Health, Zhengzhou University) Wu, Wei-Dong (Department of Toxicology, School of Public Health, Xinxiang Medical University) Yao, Wu (Department of Occupational and Environmental Health, College of Public Health, Zhengzhou University) Wu, Yong-Jun (Department of Toxicology, College of Public Health, Zhengzhou University) Li, Zhi-Tao (Department of Occupational and Environmental Health, College of Public Health, Zhengzhou University) Zhao, Yong (Department of Occupational and Environmental Health, College of Public Health, Zhengzhou University) Liu, Yu (Department of Occupational and Environmental Health, College of Public Health, Zhengzhou University) Feng, Fei-Fei (Department of Toxicology, College)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제15권 제12호

발행연도: 2014.1

수록면: 4,871 - 4,876 (6page)

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Background: We sought to evaluate the role of tumor associated macrophages (TAMs) on the promotion of coal tar pitch extract (CTPE)-induced tumorigenesis of human bronchial epithelial cells (BEAS-2B) and tumor metastasis in nude mice, and related mechanisms. Materials and Methods: BEAS-2B cells were first treated with 2.4 mg/mL CTPE for 72 hours. After removal of CTPE, the cells were continuously cultured and passaged using trypsin-EDTA. THP-1 cells were used as macrophage-like cells. BEAS-2B cells under different conditions (n=6/group) were injected into the back necks of nude mice, and alterations of tumor xenograft growth, indicative of tumorigenicity, and tumor metastasis were determined. Pathological changes (tumor nests and microvascular lesions) of HE-stained tumor tissues were also evaluated. The expression of AP-1(c-Jun) in xenografts and metastatic tumors was determined using immunohistochemistry. Results: Tumor size and weight in nude mice transplanted with the mixture of CTPE-induced passage 30 BEAS-2B and THP-1 cells (2:1) were increased compared to those from the CTPE-treated BEAS-2B cells at passage 30 alone at different observation time points. Tumor metastasis to lymph nodes and liver was only detected after transplantation of a mixture the two kinds of cells. The numbers of tumor nests and microvascular lesions, and the expression levels of AP-1 (c-Jun) in tumors from the mixture of two kinds of cells were increased apparently in contrast to those in tumor from the CTPE-treated BEAS-2B cells of passage 30 alone. In addition, there was positive correlation between AP-1 (c-Jun) expression level and the number of microvascular lesions, or between AP-1 (c-Jun) expression level and tumor metastasis in these two groups. Conclusions: TAMs not only facilitate tumorigenesis transformation of CTPE-induced BEAS-2B cells, but also promote tumor growth, angiogenesis and metastasis in nude mice in vivo, which may be mediated by AP-1.

#Tumor associated macrophages #tumorigenesis #metastasis #lung cancer #coal tar pitch #AP-1

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