Ganoderma Lucidum Polysaccharides Target a Fas/Caspase Dependent Pathway to Induce Apoptosis in Human Colon Cancer Cells

Liang, Zengenni; Guo, Yu-Tong; Yi, You-Jin; Wang, Ren-Cai; Hu, Qiu-Long; Xiong, Xing-Yao

추천

검색

자료유형: 학술저널

저자정보: Liang, Zengenni (Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University) Guo, Yu-Tong (Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University) Yi, You-Jin (Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University) Wang, Ren-Cai (Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University) Hu, Qiu-Long (Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University) Xiong, Xing-Yao (Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제15권 제9호

발행연도: 2014.1

수록면: 3,981 - 3,986 (6page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Ganoderma lucidum polysaccharides (GLP) extracted from Ganoderma lucidum have been shown to induce cell death in some kinds of cancer cells. This study investigated the cytotoxic and apoptotic effect of GLP on HCT-116 human colon cancer cells and the molecular mechanisms involved. Cell proliferation, cell migration, lactate dehydrogenase (LDH) levels and intracellular free calcium levels ($[Ca^{2+}]i$) were determined by MTT, wound-healing, LDH release and fluorescence assays, respectively. Cell apoptosis was observed by scanning and transmission electron microscopy. For the mechanism studies, caspase-8 activation, and Fas and caspase-3 expression were evaluated. Treatment of HCT-116 cells with various concentrations of GLP (0.625-5 mg/mL) resulted in a significant decrease in cell viability (P< 0.01). This study showed that the antitumor activity of GLP was related to cell migration inhibition, cell morphology changes, intracellular $Ca^{2+}$ elevation and LDH release. Also, increase in the levels of caspase-8 activity was involved in GLP-induced apoptosis. Western blotting indicated that Fas and caspase-3 protein expression was up-regulated after exposure to GLP. This investigation demonstrated for the first time that GLP shows prominent anticancer activities against the HCT-116 human colon cancer cell line through triggering intracellular calcium release and the death receptor pathway.

#Apoptosis #caspase #cell migration #Fas #human colon cancer cells

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요

이 논문의 저자 정보

Liang, Zengenni

소속기관 Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural Uni