Radiation Induces Phosphorylation of STAT3 in a Dose- and Time-dependent Manner

Gao, Ling; Li, Feng-Sheng; Chen, Xiao-Hua; Liu, Qiao-Wei; Feng, Jiang-Bin; Liu, Qing-Jie; Su, Xu

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자료유형: 학술저널

저자정보: Gao, Ling (Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention) Li, Feng-Sheng (The Second Artillery General Hospital) Chen, Xiao-Hua (Department of Experimental Therapy of Acute Radiation Sickness, Beijing Institute of Radiation Medicine) Liu, Qiao-Wei (Department of Experimental Therapy of Acute Radiation Sickness, Beijing Institute of Radiation Medicine) Feng, Jiang-Bin (Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention) Liu, Qing-Jie (Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention) Su, Xu (Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제15권 제15호

발행연도: 2014.1

수록면: 6,161 - 6,164 (4page)

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Background: We have reported the radiation could activate STAT3, which subsequently promotes the invasion of A549 cells. We here explored the dose- and time-response of STAT3 to radiation and the effect of radiation on upstream signaling molecules. Materials and Methods: A549 cells were irradiated with different doses of ${\gamma}$-rays. The expression of and nucleus translocation of p-STAT3 in A549 cells were detected by immunoblotting and immunofluorescence, respectively. The level of phosphorylated EGFR was also assessed by immunoblotting, and IL-6 expression was detected by real time PCR and ELISA. Results: Radiation promoted the phosphorylation of STAT3 at Y705 in a dose- and time-dependent manner and nuclear translocation. The level of phosphorylated EGFR in A549 cells increased after radiation. In additional, the mRNA and protein levels of IL-6 in A549 cells were also up regulated by radiation. Conclusions: STAT3 is activated by radiation in a dose-and time-dependent manner, probably due to radiation-induced activation of EGFR or secretion of IL-6 in A549 cells.

#Radiation #STAT3 #phosphorylation #EGFR #IL-6

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Gao, Ling

소속기관 Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological