Down-regulation of FRα Inhibits Proliferation and Promotes Apoptosis of Cervical Cancer Cells in Vitro

Bai, Li-Xia; Ding, Ling; Jiang, Shi-Wen; Kang, Hui-Jie; Gao, Chen-Fei; Chen, Chen; Zhou, Qin; Wang, Jin-Tao

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자료유형: 학술저널

저자정보: Bai, Li-Xia (Department of Epidemiology, School of Public Health, Shanxi Medical University) Ding, Ling (Department of Epidemiology, School of Public Health, Shanxi Medical University) Jiang, Shi-Wen (Department of Biomedical Science, Mercer University School of Medicine) Kang, Hui-Jie (Department of Epidemiology, School of Public Health, Shanxi Medical University) Gao, Chen-Fei (Department of Epidemiology, School of Public Health, Shanxi Medical University) Chen, Chen (Department of Epidemiology, School of Public Health, Shanxi Medical University) Zhou, Qin (Department of Epidemiology, School of Public Health, Shanxi Medical University) Wang, Jin-Tao (Department of Epidemiology, School of Public Health, Shanxi Medical University)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제15권 제14호

발행연도: 2014.1

수록면: 5,667 - 5,672 (6page)

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Folate receptor alpha ($FR{\alpha}$) mediates folate uptake by endocytosis, and while folate is essential to DNA methylation and synthesis and may have an important role in proliferating cells. $FR{\alpha}$ is known to be expressed in rapidly proliferating cells, including many cancer cell lines, but there has been no systematic assessment of expression in cervical cancer cell lines. The aim of the present study was to evaluate the effects of $FR{\alpha}$ on proliferation and apoptosis of cervical cells and correlation mechanism. In this study, we investigated the biological function of $FR{\alpha}$ in Hela cells using RNA interference. Cell proliferation was evaluated by Cell Counting Kit-8 (CCK8) assay, while cell cycling and apoptosis were assessed by flow cytometry, mRNA levels by real time-PCR and protein levels of $FR{\alpha}$, c-Fos and c-Jun by Western blotting. The results revealed that $FR{\alpha}$ was highly expressed in Hela cells and its silencing with a small interfering RNA (siRNA) inhibited cell proliferation and induced cell apoptosis, arresting the cell cycle in G0/G1 stages while decreasing the proportion in S and G2/M stages, and suppressed the expression levels of c-Fos and c-Jun. In conclusion, the results of this study indicated that $FR{\alpha}$ down-regulation might be capable of suppressing cervical cancer cell proliferation and promoting apoptosis. It suggested that $FR{\alpha}$ might be a novel therapeutic target for cervical cancer.

#Folate receptor alpha #proliferation #cell cycle #apoptosis #c-Fos #c-Jun

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