Effect of TLR4 and B7-H1 on Immune Escape of Urothelial Bladder Cancer and its Clinical Significance

Wang, Yong-Hua; Cao, Yan-Wei; Yang, Xue-Cheng; Niu, Hai-Tao; Sun, Li-Jiang; Wang, Xin-Sheng; Liu, Jing

추천

검색

자료유형: 학술저널

저자정보: Wang, Yong-Hua (Department of Urology, The Affiliated Hospital of Qingdao University) Cao, Yan-Wei (Department of Urology, The Affiliated Hospital of Qingdao University) Yang, Xue-Cheng (Department of Urology, The Affiliated Hospital of Qingdao University) Niu, Hai-Tao (Department of Urology, The Affiliated Hospital of Qingdao University) Sun, Li-Jiang (Department of Urology, The Affiliated Hospital of Qingdao University) Wang, Xin-Sheng (Department of Urology, The Affiliated Hospital of Qingdao University) Liu, Jing (Department of Pediatrics, The Affiliated Hospital of Qingdao University)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제15권 제3호

발행연도: 2014.1

수록면: 1,321 - 1,326 (6page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Background/Aim: Toll-like receptor 4 (TLR4) and B7-H1, both normally expressed restricted to immune cells, are found to be aberrantly expressed in a majority of human tumors and may play important roles in regulation of tumor immunity. It has been shown that urothelial bladder cancer (UBC) patients can manifest tumoral immune escape which may be a potential critical factor in tumor pathogenesis and progression. However, so far, the mechanisms of UBC-related immune escape have not been clarified. The aim of this study was to investigate the effect of TLR4 and B7-H1 on immune escape of UBC. Methods: Bladder cancer T24 cells were pre-incubated with LPS and co-cultured with tumor specific CTLs. CTL cytotoxicity and apoptosis rates were measured by MTT assay and flow cytometry, respectively. The effects of an ERK inhibitor on B7-H1 expression and CTL cytotoxicity against T24 cells were also evaluated. In addition, TLR4, B7-H1 and PD-1 protein expression was analyzed by immunohistochemistry in 60 UBC specimens and 10 normal urothelia. Results: TLR4 activation protected T24 cells from CTL killing via B7-H1 overexpression. However PD98059, an inhibitor of ERK, enhanced CTL killing of T24 cells by reducing B7-H1 expression. TLR4 expression was generally decreased in UBC specimens, while B7-H1 and PD-1 were greatly overexpressed. Moreover, expression of both B7-H1 and PD-1 was significantly associated with UICC stage and WHO grade classification. Conclusions: TLR4 and B7-H1 may contribute to immune escape of UBC. Targeting B7-H1 or the ERK pathway may offer new immunotherapy strategies for bladder cancer.

#Toll-like receptor 4 #B7-H1 #urothelial bladder cancer #immune escape

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요