Quantification of Her-2/Neu Gene in Breast Cancer Patients using Real Time-Polymerase Chain Reaction (Q-PCR) and Correlation with Immunohistochemistry Findings

Abdul Murad, Nor Azian; Razak, Zuraini Abdul; Hussain, Rosniza Muhammmad; Syed Hussain, Sharifah Noor Akmal; Ching Huat, Clarence Ko; Siti Aishah, Che Md. Ali; Abdullah, Norlia; Muhammad, Rohaizak; Ibrahim, Naqiyah; Jamal, Rahman

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저자정보: Abdul Murad, Nor Azian (UKM Medical Molecular Biology Institute) Razak, Zuraini Abdul (UKM Medical Molecular Biology Institute) Hussain, Rosniza Muhammmad (UKM Medical Molecular Biology Institute) Syed Hussain, Sharifah Noor Akmal (Department of Pathology, UKM Medical Centre, Universiti Kebansaan Malaysia) Ching Huat, Clarence Ko (Department of Pathology, UKM Medical Centre, Universiti Kebansaan Malaysia) Siti Aishah, Che Md. Ali (Department of Pathology, UKM Medical Centre, Universiti Kebansaan Malaysia) Abdullah, Norlia (Department of Surgery, UKM Medical Centre, Universiti Kebansaan Malaysia) Muhammad, Rohaizak (Department of Surgery, UKM Medical Centre, Universiti Kebansaan Malaysia) Ibrahim, Naqiyah (Department of Surgery, UKM Medical Centre, Universiti Kebansaan Malaysia) Jamal, Rahman (UKM Medical Molecular Biology Institute)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제14권 제3호

발행연도: 2013.1

수록면: 1,655 - 1,659 (5page)

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Background: HER-2/neu is a proto-oncogene that encodes a transmembrane tyrosine kinase growth factor which is crucial for stimulating growth and cellular motility. Overexpression of HER-2/neu is observed in 10-35% of human breast cancers and is associated with pathogenesis, prognosis as well as response to therapy. Given the imperative role of HER-2/neu overexpression in breast cancer, it is important to determine the magnitude of amplification which may facilitate a better prognosis as well as personalized therapy in affected patients. In this study, we determined HER-2/neu protein expression by immunohistochemistry (IHC) concurrently with HER-2/neu DNA amplification by quantitative real time-polymerase chain reaction (Q-PCR). Materials and Methods: A total of 53 paired tissue samples from breast cancer patients were frozen-sectioned to characterize the tumour and normal tissues. Only tissues with 80% tumour cells were used in this study. For confirmation, Q-PCR was used to determine the HER-2/neu DNA amplification. Results: We found 20/53 (37.7%) of the tumour tissues to be positive for HER-2/neu protein overexpression using IHC. Out of these twenty, only 9/53 (17%) cases were in agreement with the Q-PCR results. The concordance rate between IHC and Q-PCR was 79.3%. Approximately 20.7% of positive IHC cases showed no HER-2/neu gene amplification using Q-PCR. Conclusion: In conclusion, IHC can be used as an initial screening method for detection of the HER-2/neu protein overexpression. Techniques such as Q-PCR should be employed to verify the IHC results for uncertain cases as well as determination of HER-2/neu gene amplification.

#Breast cancer #HER-2/neu gene quantification #real time #polymerase chain reaction #Q-PCR)

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