Simultaneous Blockage of Epidermal Growth Factor Receptor and Cyclooxygenase-2 in a Human Xenotransplanted Lung Cancer Model

Mu, Xiao-Yan; Dong, Xue-Li; Sun, Jie; Ni, Yu-Hua; Dong, Zhang; Li, Xi-Li; Sun, Er-Lian; Yi, Zhou; Li, Gao

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자료유형: 학술저널

저자정보: Mu, Xiao-Yan (Provincial Hospital Affiliated to Shandong University, Eastern Hospital Care in Department of Respiration) Dong, Xue-Li (Provincial Hospital Affiliated to Shandong University, Eastern Hospital Care in Department of Respiration) Sun, Jie (Provincial Hospital Affiliated to Shandong University, Eastern Hospital Care in Department of Respiration) Ni, Yu-Hua (Provincial Hospital Affiliated to Shandong University, Eastern Hospital Care in Department of Respiration) Dong, Zhang (Provincial Hospital Affiliated to Shandong University, Eastern Hospital Care in Department of Respiration) Li, Xi-Li (Provincial Hospital Affiliated to Shandong University, Eastern Hospital Care in Department of Respiration) Sun, Er-Lian (Provincial Hospital Affiliated to Shandong University, Eastern Hospital Care in Department of Respiration) Yi, Zhou (Provincial Hospital Affiliated to Shandong University, Eastern Hospital Care in Department of Respiration) Li, Gao (Provincial Hospital Affiliated to Shandong University, Eastern Hospital Care in Department of Respiration)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제15권 제1호

발행연도: 2014.1

수록면: 69 - 73 (5page)

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The effects of erlotinib combined with celecoxib in a lung cancer xenograft model were here explored with a focus on possible mechanisms. A xenotransplanted lung cancer model was established in nude mice using the human lung cancer cell A549 cell line and animals demonstrating tumour growth were randomly divided into four groups: control, erlotinib, celecoxib and combined (erotinib and celecoxib). The tumor major axis and short diameter were measured twice a week and after 40 days tissues were collected for immunohistochemical analyses of Bcl-2 and Bax positive cells and Western-blotting analyses for the epidermal growth factor recepto (EGFR), P-EGFR, and cyclooxygenase-2 (COX-2). Tumor size in the combined group was smaller than in the others (p<0.01) and the percentage of Bcl-2 positive cells was fewer in most cases (p<0.01), while that of Bax positive cells was greater than in the erlotinib and celecoxib groups (P>0.05). Western blotting showed decreased expression of P-EGFR and COX-2 with both erlotinib and celecoxib treatments, but most pronouncedly in the combined group (P<0.05). Simultaneous blockage of the EGFR and COX-2 signal pathways exerted stronger growth effects in our human xenotransplanted lung cancer model than inhibition of either pathway alone. The anti-tumor effects were accompanied by synergetic inhibition of tumor cell apoptosis, activation of p-EGFR and expression of COX-2.

#Lung cancer #erlotinib #celecoxib #combination therapy

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Mu, Xiao-Yan

소속기관 Provincial Hospital Affiliated to Shandong University, Eastern Hospital Care in Department of Respir