Screening for MiRNAs Related to Laryngeal Squamous Carcinoma Stem Cell Radiation

Huang, Chang-Xin; Zhu, Ying; Duan, Guang-Liang; Yao, Ji-Fen; Li, Zhao-Yang; Li, Da; Wang, Qing-Qing

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자료유형: 학술저널

저자정보: Huang, Chang-Xin (Department of Oncology, Affiliated Hospital with Hangzhou Normal University School of Medicine) Zhu, Ying (Second Clinical Medical College of Zhejiang Chinese Medicine University) Duan, Guang-Liang (Department of Oncology, Affiliated Hospital with Hangzhou Normal University School of Medicine) Yao, Ji-Fen (Department of Oncology, Affiliated Hospital with Hangzhou Normal University School of Medicine) Li, Zhao-Yang (Department of Oncology, Affiliated Hospital with Hangzhou Normal University School of Medicine) Li, Da (Institute of Immunology, Zhejiang University) Wang, Qing-Qing (Institute of Immunology, Zhejiang University)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제14권 제8호

발행연도: 2013.1

수록면: 4,533 - 4,537 (5page)

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Objective: To use microarray chip technology for screening of stem cell radiation related miRNAs in laryngeal squamous cell carcinoma; study and explore the relationship of miRNAs with radiosensitivity of laryngeal squamous cells. Method: After conventional culture and amplification of the laryngeal squamous carcinoma cell line Hep-2, CD 133+ cells were screened out with combination of isolated culture of stem cell microspheres and FACS for preparation of laryngeal cancer stem cells. After radiation treatment, miRNAs of laryngeal squamous carcinoma stem cells before and after radiation were enriched and purified. After microarray hybridization with mammalian miRNA and scanning of fluorescence signal, the miRNAs of laryngeal squamous carcinoma stem cells before and after radiation was subject to differential screening and clustering analysis. Real-time quantitative RT-PCR was used to verify part of the differentially expressed miRNAs. Results: 70 miRNAs related to laryngeal cancer stem cell radiation with 2-fold difference in expression were screened out, in which 62 were down-regulated and 8 were up-regulated. Fluorescent quantitative RT-PCR results were consistent with miRNAs chip results. Conclusion: Some miRNAs may be involved in self-regulation with laryngeal squamous carcinoma stem cell radiation.

#Laryngeal carcinoma stem cells #miRNA #radiation related #differential expression

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Huang, Chang-Xin

소속기관 Department of Oncology, Affiliated Hospital with Hangzhou Normal University School of Medicine