Bcl-2 Gene Expression in Human Breast Cancers in Iran

Rostamizadeh, Leila; Fakhrjou, Ashraf; Montazeri, Vahid; Estiar, Mehrdad Asghari; Naghavi-Behzad, Mohammad; Hosseini, Somayyeh; Sakhinia, Masoud; Sakhinia, Ebrahim

추천

검색

자료유형: 학술저널

저자정보: Rostamizadeh, Leila (Biotechnology Research Center, Tabriz University of Medical Sciences) Fakhrjou, Ashraf (Department of Pathology, Tabriz University of Medical Sciences) Montazeri, Vahid (Department of Thoracic Surgery, Tabriz University of Medical Sciences) Estiar, Mehrdad Asghari (Students' Scientific Research Center, Tehran University of Medical Sciences) Naghavi-Behzad, Mohammad (Medical Philosophy and History Research Center, Tabriz University of Medical Sciences) Hosseini, Somayyeh (Genetics and Genomics Department, UAB) Sakhinia, Masoud (Faculty of Medicine, University of Liverpool) Sakhinia, Ebrahim (Biotechnology Research Center, Tabriz University of Medical Sciences)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제14권 제7호

발행연도: 2013.1

수록면: 4,209 - 4,214 (6page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Background: Breast cancer is among the five most common cancers and ranks first among cancers diagnosed in Iranian women. Screening and treatment of this disease with molecular methods, especially regarding high incidences at early age and advanced stage, is essential. Several genes with altered expression have been identified by cDNA microarray studies in breast cancer, with the Bcl-2 gene indicated as a likely candidate. In this study, we studied Bcl-2 gene expression levels in parallel tumor and non-tumor breast tissues. Materials and Methods: Forty samples including 21 tumor, 16 non tumor (marginal) and 3 benign breast tissues which were all pathologically diagnosed, were subjected to RNA extraction and polyA RT-PCR with the expression level of Bcl-2 quantified using real-time PCR. Results: There is higher expression levels of the Bcl-2 gene in tumor samples compared with marginal samples, but not attaining significance(p>0.05). Bcl-2 expression in 14 (66.7%) of the cases of tumor samples and 9 (56.3%) cases of the marginal samples were positive. Comparison of the expression of the Bcl-2 gene in histological grade showed that a high expression of Bcl-2 was associated with a high histological grade (p<0.41). Conclusions: Our data suggests that dysregulated Bcl-2 gene expression is potentially involved in the pathogenesis of breast cancer. Using gene expression analysis may significantly improve our ability for screening cancer patients and will prove a powerful tool in the diagnosis and prognostic evaluation of the disease whilst aiding the cooperative group trials in the Bcl-2 based therapy project.

#Breast Cancer #Bcl-2 #over expression #real-time quantitative PCR

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요