Screening of Differentially Expressed Genes among Various TNM Stages of Lung Adenocarcinoma by Genomewide Gene Expression Profile Analysis

Liu, Ming; Pan, Hong; Zhang, Feng; Zhang, Yong-Biao; Zhang, Yang; Xia, Han; Zhu, Jing; Fu, Wei-Ling; Zhang, Xiao-Li

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자료유형: 학술저널

저자정보: Liu, Ming (Cardiothoracic Surgery Department, Affiliated Daping Hospital) Pan, Hong (Department of Clinical Laboratory Sciences, Affiliated South-West Hospital, Third Military Medical University) Zhang, Feng (Beijing Institute of Genomics of the Chinese Academy of Sciences, Beijing Genomics Institute, Beijing Proteomics Institute) Zhang, Yong-Biao (Beijing Institute of Genomics of the Chinese Academy of Sciences, Beijing Genomics Institute, Beijing Proteomics Institute) Zhang, Yang (Department of Clinical Laboratory Sciences, Affiliated South-West Hospital, Third Military Medical University) Xia, Han (Department of Clinical Laboratory Sciences, Affiliated South-West Hospital, Third Military Medical University) Zhu, Jing (Department of Clinical Laboratory, the First Hospital Affiliated to the Chinese PLA General Hospital) Fu, Wei-Ling (Department of Clinical Laboratory Sciences, Affiliated South-West Hospital, Third Military Medical University) Zhang, Xiao-Li (Department of Clinical Laboratory Sciences, Affiliated South-West Hospital, Third Military Medical University)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제14권 제11호

발행연도: 2013.1

수록면: 6,281 - 6,286 (6page)

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Background: To further investigate the molecular basis of lung cancer development, we utilize a microarray to identify differentially expressed genes associated with various TNM stages of adenocarcinoma, a subtype with increasing incidence in recent years in China. Methods: A 35K oligo gene array, covering about 25,100 genes, was used to screen differentially expressed genes among 90 tumor samples of lung adenocarcinoma in various TNM stages. To verify the gene array data, three genes (Zimp7, GINS2 and NAG-1) were confirmed by real-time RT-PCR in a different set of samples from the gene array. Results: First, we obtained 640 differentially expressed genes in lung adenocarcinomas compared to the surrounding normal lung tissues. Then, from the 640 candidates we identified 10 differentially expressed genes among different TNM stages (Stage I, II and IIIA), of which Zimp7, GINS2 and NAG-1 genes were first reported to be present at a high level in lung adenocarcinoma. The results of qRT-PCR for the three genes were consistent with those from the gene array. Conclusions: We identified 10 candidate genes associated with different TNM stages in lung adenocarcinoma in the Chinese population, which should provide new insights into the molecular basis underlying the development of lung adenocarcinoma and may offer new targets for the diagnosis, therapy and prognosis prediction.

#Lung adenocarcinoma #TNM stage #gene expression profile #differential gene expression

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Liu, Ming

소속기관 Department of Radiation Oncology, Shandong Cancer Hospital and Institute, School of Medicine and Lif