Matrix Metalloproteinase-2 (-1306 C>T) Promoter Polymorphism and Risk of Colorectal Cancer in the Saudi Population

Saeed, Hesham Mahmoud; Alanazi, Mohammad Saud; Parine, Narasimha Reddy; Shaik, Jilani; Semlali, Abdelhabib; Alharbi, Othman; Azzam, Nahla; Aljebreen, Abdulrahman; Almadi, Majid; Shalaby, Manal Aly

추천

검색

자료유형: 학술저널

저자정보: Saeed, Hesham Mahmoud (Biochemistry Department, Division of Gastroenterology, Faculty of Medicine, King Khalid University Hospital) Alanazi, Mohammad Saud (Biochemistry Department, Division of Gastroenterology, Faculty of Medicine, King Khalid University Hospital) Parine, Narasimha Reddy (Biochemistry Department, Division of Gastroenterology, Faculty of Medicine, King Khalid University Hospital) Shaik, Jilani (Biochemistry Department, Division of Gastroenterology, Faculty of Medicine, King Khalid University Hospital) Semlali, Abdelhabib (Biochemistry Department, Division of Gastroenterology, Faculty of Medicine, King Khalid University Hospital) Alharbi, Othman (Department of Internal Medicine, Division of Gastroenterology, Faculty of Medicine, King Khalid University Hospital) Azzam, Nahla (Department of Internal Medicine, Division of Gastroenterology, Faculty of Medicine, King Khalid University Hospital) Aljebreen, Abdulrahman (Department of Internal Medicine, Division of Gastroenterology, Faculty of Medicine, King Khalid University Hospital) Almadi, Majid (Department of Internal Medicine, Division of Gastroenterology, Faculty of Medicine, King Khalid) Shalaby, Manal Aly

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제14권 제10호

발행연도: 2013.1

수록면: 6,025 - 6,030 (6page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Background: Matrix metalloproteinase-2 (MMP-2) is an enzyme with proteolytic activity against matrix proteins, particularly basement membrane constituents. A single nucleotide polymorphism (SNP) at -1306, which disrupts a Sp1-type promoter site (CCACC box), results in strikingly lower promoter activity with the T allele. In the present study, we investigated whether this MMP-2 genetic polymorphism might be associated with susceptibility to colorectal cancer (CRC) in the Saudi population. We also analyzed MMP-2 gene expression level sin CRC patients and 4 different cancer cell lines. Materials and Methods: TaqMan allele discrimination assays and DNA sequencing techniques were used to investigate the $C^{-1306}T$ SNP in the MMP-2 gene of Saudi colorectal cancer patients and controls. The MMP-2 gene expression level was also determined in 12 colon cancer tissue samples collected from unrelated patients and histologically normal tissues distant from tumor margins. Results and Conclusions: The MMP-2 $C^{-1306}T$ SNP in the promoter region was associated with CRC in our Saudi population and the MMP-2 gene expression level was found to be 10 times higher in CRC patients. The MMP-2 $C^{-1306}T$ SNP is significantly associated with CRC in the Saudi population and this finding suggested that MMP-2 variants might help predict CRC progression risk among Saudis. We propose that analysis of this gene polymorphism could assist in identification of patient subgroups at risk of a poor disease outcome.

#Colorectal cancer #matrix metalloproteinases #single nucleotide polymorphism #ORs #odds ratios

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요