Inhibitory Effects of β-Cyclodextrin-Helenalin Complexes on H-TERT Gene Expression in the T47D Breast Cancer Cell Line - Results of Real Time Quantitative PCR

Ghasemali, Samaneh; Nejati-Koshki, Kazem; Akbarzadeh, Abolfazl; Tafsiri, Elham; Zarghami, Nosratollah; Rahmati-Yamchi, Mohamad; Alizadeh, Effat; Barkhordari, Amin; Tozihi, Majid; Kordi, Shirafkan

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자료유형: 학술저널

저자정보: Ghasemali, Samaneh (Drug Applied Research Center, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences) Nejati-Koshki, Kazem (Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences) Akbarzadeh, Abolfazl (Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences) Tafsiri, Elham (Biotechnology Research Center, Molecular Medicine Department, Pasteur Institute of Iran) Zarghami, Nosratollah (Drug Applied Research Center, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences) Rahmati-Yamchi, Mohamad (Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences) Alizadeh, Effat (Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences) Barkhordari, Amin (Drug Applied Research Center, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences) Tozihi, Majid (Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences) Kordi, Shirafkan (Department)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제14권 제11호

발행연도: 2013.1

수록면: 6,949 - 6,953 (5page)

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Background: Nowadays, the encapsulation of cytotoxic chemotherapeutic agents is attracting interest as a method for drug delivery. We hypothesized that the efficiency of helenalin might be maximized by encapsulation in ${\beta}$-cyclodextrin nanoparticles. Helenalin, with a hydrophobic structure obtained from flowers of Arnica chamissonis and Arnica Montana, has anti-cancer and anti-inflammatory activity but low water solubility and bioavailability. ${\beta}$-Cyclodextrin (${\beta}$-CD) is a cyclic oligosaccharide comprising seven D-glucopyranoside units, linked through 1,4-glycosidic bonds. Materials and Methods: To test our hypothesis, we prepared ${\beta}$-cyclodextrin-helenalin complexes to determine their inhibitory effects on telomerase gene expression by real-time polymerase chain reaction (q-PCR) and cytotoxic effects by colorimetric cell viability (MTT) assay. Results: MTT assay showed that not only ${\beta}$-cyclodextrin has no cytotoxic effect on its own but also it demonstrated that ${\beta}$-cyclodextrin-helenalin complexes inhibited the growth of the T47D breast cancer cell line in a time and dose-dependent manner. Our q-PCR results showed that the expression of telomerase gene was effectively reduced as the concentration of ${\beta}$-cyclodextrin-helenalin complexes increased. Conclusions: ${\beta}$-Cyclodextrin-helenalin complexes exerted cytotoxic effects on T47D cells through down-regulation of telomerase expression and by enhancing Helenalin uptake by cells. Therefore, ${\beta}$-cyclodextrin could be superior carrier for this kind of hydrophobic agent.

#Helenalin #Telomerase #real time quantitative PCR #${beta}$-cyclodextrin

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Ghasemali, Samaneh

소속기관 Drug Applied Research Center, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sci