Synergistic Effects of Tamoxifen and Tranilast on VEGF and MMP-9 Regulation in Cultured Human Breast Cancer Cells

Darakhshan, Sara; Bidmeshkipour, Ali; Khazaei, Mozafar; Rabzia, Arezou; Ghanbari, Ali

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자료유형: 학술저널

저자정보: Darakhshan, Sara (Department of Biology, Faculty of Science, Razi University) Bidmeshkipour, Ali (Department of Biology, Faculty of Science, Razi University) Khazaei, Mozafar (Fertility and Infertility Research Center, Kermanshah University of Medical Sciences) Rabzia, Arezou (Fertility and Infertility Research Center, Kermanshah University of Medical Sciences) Ghanbari, Ali (Fertility and Infertility Research Center, Kermanshah University of Medical Sciences)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제14권 제11호

발행연도: 2013.1

수록면: 6,869 - 6,874 (6page)

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Background: Vascular endothelial growth factor and matrix metalloproteinases are two important factors for angiogenesis associated with breast cancer growth and progression. The present study was aimed to examine the effects of tamoxifen and tranilast drugs singly or in combination on proliferation of breast cancer cells and also to evaluate VEGF and MMP-9 expression and VEGF secretion levels. Materials and Methods: Human breast cancer cell lines, MCF-7 and MDA-MB-231, were treated with tamoxifen and/or tranilast alone or in combination and percentage cell survival and proliferative activity were evaluated using LDH leakage and MTT assays. mRNA expression and protein levels were examined by real-time RT-PCR and ELISA assay, respectively. Results: LDH and MTT assays showed that the combined treatment of tamoxifen and tranilast resulted in a significant decrease in cell viability and cell proliferation compared with tamoxifen or tranilast treatment alone, with significant decrease in VEGF mRNA and protein levels. We also found that tamoxifen as a single agent rarely increased MMP-9 expression. A decrease in MMP-9 expression was seen after treatment with tranilast alone and in the combined treatment MMP-9 mRNA level was decreased. Conclusions: This combination treatment can able to inhibit growth, proliferation and angiogenesis of breast cancer cells.

#Breast cancer #tamoxifen #tranilast #angiogenesis #matrix metalloproteinase

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