Anti-proliferative Effects of Atractylis lancea (Thunb.) DC. via Down-regulation of the c-myc/hTERT/Telomerase Pathway in Hep-G2 Cells

Guo, Wei-Qiang; Li, Liang-Zhi; He, Zhuo-Yang; Zhang, Qi; Liu, Jia; Hu, Cui-Ying; Qin, Fen-Ju; Wang, Tao-Yun

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자료유형: 학술저널

저자정보: Guo, Wei-Qiang (School of Chemistry, Biology and Material Engineering, Suzhou University of Science and Technology) Li, Liang-Zhi (School of Chemistry, Biology and Material Engineering, Suzhou University of Science and Technology) He, Zhuo-Yang (School of Chemistry, Biology and Material Engineering, Suzhou University of Science and Technology) Zhang, Qi (School of Chemistry, Biology and Material Engineering, Suzhou University of Science and Technology) Liu, Jia (School of Chemistry, Biology and Material Engineering, Suzhou University of Science and Technology) Hu, Cui-Ying (School of Chemistry, Biology and Material Engineering, Suzhou University of Science and Technology) Qin, Fen-Ju (School of Chemistry, Biology and Material Engineering, Suzhou University of Science and Technology) Wang, Tao-Yun (School of Chemistry, Biology and Material Engineering, Suzhou University of Science and Technology)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제14권 제11호

발행연도: 2013.1

수록면: 6,363 - 6,367 (5page)

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Atractylis lancea (Thunb.) DC. (AL), an important medicinal herb in Asia, has been shown to have anti-tumor effects on cancer cells, but the involved mechanisms are poorly understood. This study focused on potential effects and molecular mechanisms of AL on the proliferation of the Hep-G2 liver cancer cell line in vitro. Cell viability was assessed by MTT test in Hep-G2 cells incubated with an ethanol extract of AL. Then, the effects of AL on apoptosis and cell cycle progression were determined by flow cytometry. Telomeric repeat amplification protocol (TRAP) assays was performed to investigate telomerase activity. The mRNA and protein expression of human telomerase reverse transcriptase (hTERT) and c-myc were determined by real-time RT-PCR and Western blotting. Our results show that AL effectively inhibits proliferation in Hep-G2 cells in a concentrationand time-dependent manner. When Hep-G2 cells were treated with AL after 48h,the $IC_{50}$ was about 72.1 ${\mu}g/mL$. Apoptosis was induced by AL via arresting the cells in the G1 phase. Furthermore, AL effectively reduced telomerase activity through inhibition of mRNA and protein expression of hTERT and c-myc. Hence, these data demonstrate that AL exerts anti-proliferative effects in Hep-G2 cells via down-regulation of the c-myc/hTERT/telomerase pathway.

#Atractylis lancea #Thunb #) DC #liver cancer #telomerase activity #hTERT #C-myc

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Guo, Wei-Qiang

소속기관 School of Chemistry, Biology and Material Engineering, Suzhou University of Science and Technology