Comparative Proteomics Analysis of Colorectal Cancer

Wang, Jun-Jiang; Liu, Ying; Zheng, Yang; Lin, Feng; Cai, Guan-Fu; Yao, Xue-Qing

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저자정보: Wang, Jun-Jiang (Department of Gastrointestinal Surgery, Guangdong Academy of Medical Sciences, Guangdong General Hospital) Liu, Ying (Department of Obstetrics and Gynecology, Guangdong Academy of Medical Sciences, Guangdong General Hospital) Zheng, Yang (Department of Gastroenterology Surgery, the First Affiliated Hospital of Shan-tou University Medical College) Lin, Feng (Department of Gastrointestinal Surgery, Guangdong Academy of Medical Sciences, Guangdong General Hospital) Cai, Guan-Fu (Department of Gastrointestinal Surgery, Guangdong Academy of Medical Sciences, Guangdong General Hospital) Yao, Xue-Qing (Department of Gastrointestinal Surgery, Guangdong Academy of Medical Sciences, Guangdong General Hospital)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제13권 제4호

발행연도: 2012.1

수록면: 1,663 - 1,666 (4page)

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Background and Objective: Protein expression in colon and rectal cancer (CRC) and paired normal tissues was examined by two-dimensional gel electrophoresis (2-DE) to identify differentially expressed proteins. Materials and Methods: Five fresh colorectal cancer and paired adjacent normal tissues were obtained and differentially expressed protein spots were determined using PDQuest software, with identification on the basis of MALDI-TOF mass spectra. Results: Compared with normal colorectal mucosa, protein abnormal expression of 65 spots varying more than 1.5 times were found in 2-DE gels from colorectal cancer samples (P<0.05); forty-two proteins were up-regulated and 23 were down-regulated; twelve protein spots were identified using mass spectrometry, of which 8 were up-regulated, includimng HSPB1and Annexin A4, while 4 were down-regulated, the results being consistent with Western blot findings. Conclusions: Two-dimensional electrophoresis reference maps for CRC tissues and adjacent normal mucosa (NMC) were established and 12 differentially expressed proteins were identified. Up-regulated HSPB1 and Annexin A4 may play many important roles in the pathogenesis of colorectal cancer.

#Colorectal cancer #two-dimensional gel electrophoresis #mass spectrometry

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Wang, Jun-Jiang

소속기관 Department of Gastrointestinal Surgery, Guangdong Academy of Medical Sciences, Guangdong General Hos