Experimental Study of Endostar Injection Concomitant with Cryoablation on Lung Adenocarcinoma A549 Xenografts

Ma, Chun-Hua; Jiang, Rong; Li, Jin-Duo; Wang, Bin; Sun, Li-Wei; Lv, Yuan

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자료유형: 학술저널

저자정보: Ma, Chun-Hua (Department of Intervention, Tianjin Huanhu Hospital, Tianjin Cerebral Vascular and Neural Degenerative Disease Key Laboratory) Jiang, Rong (Department of Intervention, Tianjin Huanhu Hospital, Tianjin Cerebral Vascular and Neural Degenerative Disease Key Laboratory) Li, Jin-Duo (Department of Intervention, Tianjin Huanhu Hospital, Tianjin Cerebral Vascular and Neural Degenerative Disease Key Laboratory) Wang, Bin (Department of Intervention, Tianjin Huanhu Hospital, Tianjin Cerebral Vascular and Neural Degenerative Disease Key Laboratory) Sun, Li-Wei (Department of Intervention, Tianjin Huanhu Hospital, Tianjin Cerebral Vascular and Neural Degenerative Disease Key Laboratory) Lv, Yuan (Department of Intervention, Tianjin Huanhu Hospital, Tianjin Cerebral Vascular and Neural Degenerative Disease Key Laboratory)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제14권 제11호

발행연도: 2013.1

수록면: 6,697 - 6,701 (5page)

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Objective: To explore the inhibiting effect and mechanism of Endostar injection concomitant with cryoablation on lung adenocarcinoma A549 xenografts in nude mice. Materials and Methods: A total of 24 nude mice with subcutaneous xenografts of the A549 cell line were established and divided into 4 groups when the maximal diameters of tumors became 1 cm: control group, Endostar group, cryoablation group and combination group (Endostar concomitant with cryoablation). The nude mice were sacrificed after 21-days treatment, tumour tissues were removed to measure their volume, in situ test of TdT-mediated dUTP nick end labeling (TUNEL) was adopted to determine the cellular apoptosis around freezing injury zones, and immunohistochemical SP test was applied for the detection of micro-vessel density (MVD) and vascular endothelial growth factor (VEGF) expression levels. Results: At 21-days after treatment, the growth velocities of control group, Endostar group, cryoablation group and combination group were $236.7{\pm}51.2%$, $220.0{\pm}30.6%$, $159.5{\pm}29.3%$ and $103.3{\pm}25.5%$ (P<0.01), while cellular apoptosis rates of tumors were $21.7{\pm}2.34%$, ($22.17{\pm}1.47$)%, $38.3{\pm}1.37%$ and $49.2{\pm}1.72%$, (P<0.01), respectively, according to the immunohistochemical test. MVD and VEGF expression levels in the combination group were both lower than in other groups (P<0.01), also being positively related (r=0.925, P<0.01). Conclusions: Endostar can significantly improve the inhibitory effects of cryoablation on xenografts of lung adenocarcinoma A549, and the mechanism is probably associated with its function as an inhibitor of tumour neo-angiogenesis through down-regulating VEGF expression.

#Endostar #cryoablation #lung adenocarcinoma A549 xenografts #VEGF #micro-vessel density

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Ma, Chun-Hua

소속기관 Department of Intervention, Tianjin Huanhu Hospital, Tianjin Cerebral Vascular and Neural Degenerati