Chemopreventive Potential of an Ethyl Acetate Fraction from Curcuma Longa is Associated with Upregulation of p57 and Rad9 in the PC-3M Prostate Cancer Cell Line

Rao, K.V.K.; Samikkannu, T.; Dakshayani, K.B.; Zhang, X.; Sathaye, S.S.; Indap, M.A.; Nair, Madhavan P.N.

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자료유형: 학술저널

저자정보: Rao, K.V.K. (Department of Immunology, College of Medicine, Florida international University) Samikkannu, T. (Department of Immunology, College of Medicine, Florida international University) Dakshayani, K.B. (Department of Immunology, College of Medicine, Florida international University) Zhang, X. (Department of Chemistry, Boston University) Sathaye, S.S. (Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology) Indap, M.A. (Chemotherapy Division [Retd.], ACTREC) Nair, Madhavan P.N. (Department of Immunology, College of Medicine, Florida international University)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제13권 제3호

발행연도: 2012.1

수록면: 1,031 - 1,038 (8page)

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Background: Turmeric ($Curcuma$ $longa$) has been shown to possess anti-inflammatory, antioxidant and antitumor properties. However, despite the progress in research with $C.$ $longa$, there is still a big lacuna in the information on the active principles and their molecular targets. More particularly very little is known about the role of cell cycle genes $p57^{kip2}$ and Rad9 during chemoprevention by turmeric and its derivatives especially in prostate cancer cell lines. Methods: Accordingly, in this study, we have examined the antitumor effect of several extracts of $C.$ $longa$ rhizomes by successive fractionation in clonogenic assays using highly metastatic PC-3M prostate cancer cell line. Results: A mixture of isopropyl alcohol: acetone: water: chloroform: and methanol extract of $C.$ $longa$ showed significant bioactivity. Further partition of this extract showed that bioactivity resides in the dichloromethane soluble fraction. Column chromatography of this fraction showed presence of biological activity only in ethyl acetate eluted fraction. HPLC, UV-Vis and Mass spectra studies showed presence three curcuminoids in this fraction besides few unidentified components. Conclusions: From these observations it was concluded that the ethyl acetate fraction showed not only inhibition of colony forming ability of PC-3M cells but also up-regulated cell cycle genes $p57^{kip2}$ and Rad9 and further reduced the migration and invasive ability of prostate cancer cells.

#Curcuma longa #ethyl acetate fraction #up-regulation #$p57^{kip2}$ and Rad9 genes #invasive ability

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