Aberrant DNA Methylation of P16, MGMT, and hMLH1 Genes in Combination with MTHFR C677T Genetic Polymorphism and Folate Intake in Esophageal Squamous Cell Carcinoma

Chen, Jing; Huang, Zhi-Jie; Duan, Yu-Qin; Xiao, Xin-Rong; Jiang, Jian-Qing; Zhang, Ru

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자료유형: 학술저널

저자정보: Chen, Jing (Department of Cadre Ward, the General Hospital of Chengdu Military Area) Huang, Zhi-Jie (Department of Nuclear Medicine, the General Hospital of Chengdu Military Area) Duan, Yu-Qin (Department of Cadre Ward, the General Hospital of Chengdu Military Area) Xiao, Xin-Rong (Department of Cadre Ward, the General Hospital of Chengdu Military Area) Jiang, Jian-Qing (Gastrointestinal Surgery, the General Hospital of Chengdu Military Area) Zhang, Ru (Department of Cadre Ward, the General Hospital of Chengdu Military Area)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제13권 제10호

발행연도: 2012.1

수록면: 5,303 - 5,306 (4page)

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Aim: The present case-control study was conducted to explore the association of MTHFR gene polymorphism and relations of P16, MGMT and HMLH1 to MTHFR and folate intake. Methods: A total of 257 cases of esophageal squamous cell carcinoma confirmed by histopathological examination were collected. Genotyping of P16, MGMT and HMLH1 was accomplished by methylation-specific polymerase chain reaction (PCR) after sodium bisulfate modification of DNA and the MTHFR C677T genetic polymorphism was detected by PCR-restriction fragment-length polymorphism (PCR-RFLP). Results: The proportions of DNA hypermethylation in P16, MGMT and hMLH1 in cancer tissues were significantly higher than in paracancerous normal tissue. The proportion of hypermethylation in at least one gene was 88.5% in cancer tissue, and was also significantly higher than that in paracancerous normal tissue. Our finding showed individuals with homozygotes (TT) of MTHFR C677T had significant risk of DNA hypermethylation of MGMT in cancer tissues, with an OR (95% CI) of 3.15 (1.12-6.87). Similarly, patients with high intake of folate also showed a slight high risk of DNA methylation of MGMT, with OR (95% CI) of 2.03 (1.05-4.57). Conclusion: Our study found the P16, MGMT and hMLH1 demonstrate a high proportion of hypermethylation in esophageal squamous cell cancer cancer tissues, which might be used as biomarkers for cancer detection.

#P16 #DNA methylation #MGMT #hMLH1 #MTHFR #esophageal squamous cell carcinoma

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Chen, Jing

소속기관 Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Science