Bracken-fern Extracts Induce Cell Cycle Arrest and Apoptosis in Certain Cancer Cell Lines

Roudsari, Motahhareh Tourchi; Bahrami, Ahmad Reza; Dehghani, Hesam; Iranshahi, Mehrdad; Matin, Maryam Moghadam; Mahmoudi, Mahmud

추천

검색

자료유형: 학술저널

저자정보: Roudsari, Motahhareh Tourchi (Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad) Bahrami, Ahmad Reza (Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad) Dehghani, Hesam (Embryonic and Stem Cell Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad) Iranshahi, Mehrdad (Biotechnology Research Center and School of Pharmacy, Mashhad University of Medical Sciences) Matin, Maryam Moghadam (Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad) Mahmoudi, Mahmud (Immunology Research Center of Bu Ali, Mashhad University of Medical Sciences)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제13권 제12호

발행연도: 2012.1

수록면: 6,047 - 6,053 (7page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Bracken fern [Pteridium aquilinem (L.) kuhn (Dennstaedtiaceae)] is one of the most common species on the planet. It has been consumed by humans and animals for centuries. Use by some human groups is because they believe bracken fern is good for health as plant medicine. However, it is also one of the few known plants that can cause tumors in farm animals. Many interested groups have focused their attention on bracken fern because of these interesting features. In order to evaluate the biological effects of exposure to this plant in cellular level, human cancer cell lines were treated with the fern dichloromethane extracts and the genotoxic and cytotoxic effects were studied. Anti-proliferative/cytotoxic effects were evaluated by cell count, MTT assay and flow cytometry methods with three different cancer cell lines, TCC, NTERA2, and MCF-7, and two normal cells, HDF1 and HFF3. Pro-apoptotic effects of the extracts were determined by DAPI staining and comet assay, on TCC cancer cells compared to the normal control cell lines. Cellular morphology was examined by light microscopy. Our present study showed that the extract caused DNA damage and apoptosis at high concentrations ($200{\mu}g/mL$) and also it may induce cell cycle arrest (G2/M phase) at mild concentrations (50 and $30{\mu}g/mL$) depending on the cell type and tumor origin. These results indicate that bracken fern extract is a potent source of anticancer compounds that could be utilized pharmaceutically.

#Bracken ferns #cell proliferation #apoptosis #cell cycle arrest

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요