Synaptic Vesicle Protein 2 (SV2) Isoforms

Bandala, Cindy; Miliar-Garcia, A.; Mejia-Barradas, C.M.; Anaya-Ruiz, M.; Luna-Arias, J.P.; Bazan-Mendez, C.I.; Gomez-Lopez, M.; Juarez-Mendez, S.; Lara-Padilla, E.

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자료유형: 학술저널

저자정보: Bandala, Cindy (Research Support Group, National Institute of Rehabilitation, Escuela Superior de Medicina, Instituto Politecnico Nacional) Miliar-Garcia, A. (Oncology and Oxidative Stress Laboratory, Escuela Superior de Medicina, Instituto Politecnico Nacional) Mejia-Barradas, C.M. (Research Support Group, National Institute of Rehabilitation, Escuela Superior de Medicina, Instituto Politecnico Nacional) Anaya-Ruiz, M. (Molecular Biology Laboratory, Escuela Superior de Medicina, Instituto Politecnico Nacional) Luna-Arias, J.P. (Cell Biology Laboratory, Eastern Biomedical Research Center, Instituto Mexicano del Seguro Social) Bazan-Mendez, C.I. (Cell Biology Laboratory, Eastern Biomedical Research Center, Instituto Mexicano del Seguro Social) Gomez-Lopez, M. (Oncology and Oxidative Stress Laboratory, Escuela Superior de Medicina, Instituto Politecnico Nacional) Juarez-Mendez, S. (Cell Biology Laboratory, CINVESTAV) Lara-Padilla, E. (Research Support Group, National Institute of Rehabilitation, Escuela Superior de Medicina, Instituto Politecnico Nacional)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제13권 제10호

발행연도: 2012.1

수록면: 5,063 - 5,067 (5page)

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New molecular markers of cancer had emerged with novel applications in cancer prevention and therapeutics, including for breast cancer of unknown causes, which has a high impact on the health of women worldwide. The purpose of this research was to detemine protein and mRNA expression of synaptic vesicle 2 (SV2) isoforms A, B and C in breast cancer cell lines. Cultured cell lines MDA-MB-231, SKBR3, T47D were lysed and their protein and mRNA expression analyzed by real-time PCR and western blot technique, respectively. SV2A, B proteins were identified in non-tumor (MCF-10A) and tumor cell lines (MDA-MB-231 and T47D) while SV2C only was found in the T47D cell line. Furthermore, the genomic expression was consistent with protein expression for a such cell line, but in MDA-MB-231 there was no SV2B genomic expression, and the SV2C mRNA and protein were not found in the non tumoral cell line. These findings suggest a possible cellular transdifferentiation to neural character in breast cancer, of possible relevance to cancer development, and point to possible use of SV2 as molecular marker and a vehicle for cancer treatment with botulinum toxin.

#SV2A #SV2B #SV2C #breast-cancer #protein/gene expression #BoNTA

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