지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 523 - 532 (10page)
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초록· 키워드
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This study was done to evaluate the antioxidant effect of oak hot water extracts on the oxidative stress induced by reactive oxygen species (ROS). The cytotoxicity of $H_2O_2$-induced oxidative stress was performed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay for the cell viability according to the dose-dependent treatment. Oak extracts demonstrated a dose-dependent ability to inhibit $H_2O_2$-induced apoptosis in cultured tenofibroblasts, as assessed by MTT assay and FACS analysis. $H_2O_2$ increased the phosphorylation of extracellular regulated kinase1/2 (ERK1/2) and of c-Jun N-terminal kinase (JNK) and the production of reactive oxygen species (ROS). In contrast, treatment with oak extracts was decreased this activation of ERK1/2 and JNK, as confirmed by western blot analysis, and reduced the production of ROS, as verified by fluorescent microscopic and flow cytometry (FACS) analyses. These findings suggest that oak extracts, by suppressing JNK, ERK1/2, and intracellular ROS production, have a concentration-dependent antiapoptotic effect on achilles tenofibroblasts exposed to an oxidative stressor, and may have therapeutic potential.
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참고문헌 (19)
참고문헌 신청
Ballesteros, M. / 2004 / Ethanol from lignocellulosic materials by a simultaneous saccharification and fermentation process(SFS)with Kluyveromyces marxianus CECT 10875 / Process Biochemistry 39:1843~1848
Chang, L. / 2001 / Mammalian MAP kinase signalling cascades / Nature 410:37~40
Chen, J. / 2010 / In chronic lateral epicondylitis, apoptosis and autophagic cell death occur in the extensor carpi radialisbrevis tendon / Journal of Shoulder and Elbow Surgery 19:355~362
Chen, Q. M. / 1998 / Molecular analysis of H2O2-induced senescent-like growth arrest in normal human fibroblasts : p53 and Rb control G1 arrest but not cell replication / Biochemical Journal 332:43~50
Fernández de Simón, B. / 2014 / Polyphenolic compounds as chemical markers of wine ageing in contact with cherry, chestnut, false acacia, ash and oak wood / Food Chemistry 143:66~76
Chang, L. / 2001 / Mammalian MAP kinase signalling cascades / Nature 410:37~40
Chen, J. / 2010 / In chronic lateral epicondylitis, apoptosis and autophagic cell death occur in the extensor carpi radialisbrevis tendon / Journal of Shoulder and Elbow Surgery 19:355~362
Chen, Q. M. / 1998 / Molecular analysis of H2O2-induced senescent-like growth arrest in normal human fibroblasts : p53 and Rb control G1 arrest but not cell replication / Biochemical Journal 332:43~50
Fernández de Simón, B. / 2014 / Polyphenolic compounds as chemical markers of wine ageing in contact with cherry, chestnut, false acacia, ash and oak wood / Food Chemistry 143:66~76
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