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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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Biocatalytic degradation of total petroleum hydrocarbons (TPHs) in contaminated soil by hemoglobin and hydrogen peroxide is an effective soil remediation method. This study used a laboratory soil reactor experiment to evaluate the effectiveness of a nonspecific biocatalytic reaction with hemoglobin and H<sub>2</sub>O<sub>2</sub> for treating TPH-contaminated soil. We also quantified changes in the soil microbial community using real-time PCR analysis during the experimental treatment. The results show that the measured rate constant for the reaction with added hemoglobin was 0.051/day, about 3.5 times higher than the constant for the reaction with only H<sub>2</sub>O<sub>2</sub> (0.014/day). After four weeks of treatment, 76% of the initial soil TPH concentration was removed with hemoglobin and hydrogen peroxide treatment. The removal of initial soil TPH concentration was 26% when only hydrogen peroxide was used. The soil microbial community, based on 16S rRNA gene copy number, was higher (7.1 × 10<sup>6</sup> copy number/g of bacteria, and 7.4 × 10<sup>5</sup> copy number/g of Archaea, respectively) in the hemoglobin catalyzed treatment. Our results show that TPH treatment in contaminated soil using hemoglobin catalyzed oxidation led to the enhanced removal effectiveness and was non-toxic to the native soil microbial community in the initial soil.
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참고문헌 (16)
참고문헌 신청
Casamayor, E. / 2002 / Changes in archaeal, bacterial and eukaryal assemblages along a salinity gradient by comparison of genetic fingerprinting methods in a multipond solar saltern / Environ. Microbiol 4:338~348
Chen, S. / 1999 / Pentachlorophenol and crystal violet degradation in water and soils using heme and hydrogen peroxide / Wat. Res 33(17):3657~3665
Chen, S. / 2006 / Treating soil PCP at optimal conditions using heme and peroxide / J. Environ. Eng., ASCE 132(7):704~708
Chen, S. / 2009 / Kinetics of pentachlorophenol degradation in soil using heme and peroxide / J. Environ. Eng., ASCE 135(4):279~284
정남현 / 2014 / Verification of Heme Catalytic Cycle with 5-Aminosalicylic Acid and Its Application to Soil Remediation of Polycyclic Aromatic Hydrocarbons / Environmental Engineering Research 19(2):139~143
Chen, S. / 1999 / Pentachlorophenol and crystal violet degradation in water and soils using heme and hydrogen peroxide / Wat. Res 33(17):3657~3665
Chen, S. / 2006 / Treating soil PCP at optimal conditions using heme and peroxide / J. Environ. Eng., ASCE 132(7):704~708
Chen, S. / 2009 / Kinetics of pentachlorophenol degradation in soil using heme and peroxide / J. Environ. Eng., ASCE 135(4):279~284
정남현 / 2014 / Verification of Heme Catalytic Cycle with 5-Aminosalicylic Acid and Its Application to Soil Remediation of Polycyclic Aromatic Hydrocarbons / Environmental Engineering Research 19(2):139~143
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