지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한생식의학회 Clinical and experimental reproductive medicine : CERM Clinical and experimental reproductive medicine : CERM 제39권 제4호
- 발행연도
- 2012.1
- 수록면
- 153 - 160 (8page)
이용수
초록· 키워드
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Objective: The aim of this study was to compare vitrification optimization of mouse embryos using electron microscopy (EM) grid, cryotop, and thin plastic strip (TPS) containers by evaluating developmental competence and apoptosis rates. Methods: Mouse embryos were obtained from superovulated mice. Mouse cleavage-stage, expanded, hatching-stage, and hatched-stage embryos were cryopreserved in EM grid, cryotop, and TPS containers by vitrification in 15% ethylene glycol, 15% dimethylsulfoxide, 10 ${\mu}g/mL$ Ficoll, and 0.65 M sucrose, and 20% serum substitute supplement (SSS) with basal medium, respectively. For the three groups in which the embryos were thawed in the EM grid, cryotop, and TPS containers, the thawing solution consisted of 0.25 M sucrose, 0.125 M sucrose, and 20% SSS with basal medium, respectively. Rates of survival, re-expansion, reaching the hatched stage, and apoptosis after thawing were compared among the three groups. Results: Developmental competence after thawing of vitrified expanded and hatching-stage blastocysts using cryotop and TPS methods were significantly higher than survival using the EM grid (p<0.05). Also, apoptosis positive nuclei rates after thawing of vitrified expanded blastocysts using cryotop and TPS were significantly lower than when using the EM grid (p<0.05). Conclusion: The TPS vitrification method has the advantages of achieving a high developmental ability and effective preservation.
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참고문헌 (41)
참고문헌 신청
Rall WF / 1985 / Ice-free cryopreservation of mouse embryos at -196 degrees C by vitrification / Nature 313:573~575
Gordts S / 1990 / Survival and pregnancy outcome after ultrarapid freezing of human embryos / Fertil Steril 53:469~472
Martino A / 1996 / Development into blastocysts of bovine oocytes cryopreserved by ultra-rapid cooling / Biol Reprod 54:1059~1069
Son WY / 2003 / Pregnancy outcome following transfer of human blastocysts vitrified on electron microscopy grids after induced collapse of the blastocoele / Hum Reprod 18:137~139
Lee SY / 2006 / Optimization of a dilution method for human expanded blastocysts vitrified using EM grids after artificial shrinkage / J Assist Reprod Genet 23:87~91
Gordts S / 1990 / Survival and pregnancy outcome after ultrarapid freezing of human embryos / Fertil Steril 53:469~472
Martino A / 1996 / Development into blastocysts of bovine oocytes cryopreserved by ultra-rapid cooling / Biol Reprod 54:1059~1069
Son WY / 2003 / Pregnancy outcome following transfer of human blastocysts vitrified on electron microscopy grids after induced collapse of the blastocoele / Hum Reprod 18:137~139
Lee SY / 2006 / Optimization of a dilution method for human expanded blastocysts vitrified using EM grids after artificial shrinkage / J Assist Reprod Genet 23:87~91
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