지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한생식의학회 Clinical and experimental reproductive medicine : CERM Clinical and experimental reproductive medicine : CERM 제38권 제3호
- 발행연도
- 2011.1
- 수록면
- 142 - 147 (6page)
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초록· 키워드
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Objective: This study was performed to evaluate testicular sperm chromatin condensation using aniline blue-eosin (AB-E) staining and its effects on IVF-ET. Methods: Chromatin condensation was analyzed using AB-E staining in 27 cases of testicular sperm extraction. There were 19 cases of obstructive azoospermia (OA) and 8 cases of non-obstructive azoospermia (NOA) in IVF-ET. Mature sperm heads were stained red-pink whereas immature sperm heads were stained dark blue. The percentage of sperm chromatin condensation was calculated from the ratio of the number of red-pink sperm to the total number of sperm analyzed. Results: The overall percentages of chromatin condensation in OA and NOA were $31.1{\pm}11.2%$ and $26.3{\pm}14.4%$, respectively. The fertilization rate was significant higher in OA than NOA ($p$ <0.05); however, the rates of good embryos and clinical pregnancy did not show statistical differences. In OA and NOA, statistical differences were not observed in the rate of chromatin condensation, fertilization, good embryos, and clinical pregnancy between the pregnant group and non-pregnant group. Conclusion: Chromatin condensation is less stable than OA and showed a low fertilization rate in NOA. While there were no significant differences in chromatin condensation results between NOA and OA, we propose that a pattern of decreased chromatin condensation in NOA is one of the factors of low fertilization results requiring further study.
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등록된 정보가 없습니다.
참고문헌 (29)
참고문헌 신청
Hofmann N / 1991 / Use of aniline blue to assess chromatin condensation in morphologically normal spermatozoa in normal and infertile men / Hum Reprod 6:979~982
Sun JG / 1997 / Detection of deoxyribonucleic acid fragmentation in human sperm: correlation with fertilization in vitro / Biol Reprod 56:602~607
Spanò M / 2000 / Sperm chromatin damage impairs human fertility. The Danish First Pregnancy Planner Study Team / Fertil Steril 73:43~50
Evenson DP / 1999 / Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic / Hum Reprod 14:1039~1049
Evenson DP / 2002 / Sperm chromatin structure assay: its clinical use for detecting sperm DNA fragmentation in male infertility and comparisons with other techniques / J Androl 23:25~43
Sun JG / 1997 / Detection of deoxyribonucleic acid fragmentation in human sperm: correlation with fertilization in vitro / Biol Reprod 56:602~607
Spanò M / 2000 / Sperm chromatin damage impairs human fertility. The Danish First Pregnancy Planner Study Team / Fertil Steril 73:43~50
Evenson DP / 1999 / Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic / Hum Reprod 14:1039~1049
Evenson DP / 2002 / Sperm chromatin structure assay: its clinical use for detecting sperm DNA fragmentation in male infertility and comparisons with other techniques / J Androl 23:25~43
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